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首页> 外文期刊>Parasitology >A.a d Proteophosphoglycan is differentially expressed in sodium stibogluconate-sensitive and resistant Indian clinical isolates of Leishmania donovani
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A.a d Proteophosphoglycan is differentially expressed in sodium stibogluconate-sensitive and resistant Indian clinical isolates of Leishmania donovani

机译：A.a d蛋白磷酸聚糖在对stibogluconate敏感和耐药的印度利什曼原虫的临床分离株中差异表达

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摘要

Leishmania produce several types of mucin-like glycoproteins called proteophosphoglycans (PPGs) some of which are secreted while others are found on the surface of promastigotes and amastigotes. These proteins are thought to be important in the transmission, invasion and subsequent intracellular survival of parasites. The structure and function of PPGs are species and stage-specific in the case of L. major and L. mexicana, but no such information has hitherto been available for L. donovani. This study presents, for the first time, an initial characterization (localization) of PPG in sodium stibogluconate (SSG)-resistant and sensitive clinical isolates of L. donovani from Bihar (India) by confocal microscopy, flow cytometry and Western blotting using antibodies to L. major PPG. Confocal microscopy analysis revealed that both promastigotes and amastigotes possess PPG on their cell membrane and flagellar pocket membrane but its expression was variable in different isolates. The quantitative analysis by FACS and Western blotting showed that the expression and intensity of PPG bands was higher in SSG-resistant isolates. This study suggests the possibilities of involvement of PPG in drug-resistant mechanisms and of using PPG abundance as a marker for identifying drug-resistant clinical isolates in Indian kala azar. 2007 Cambridge University Press.
机译：利什曼原虫产生几种类型的粘蛋白样糖蛋白，称为蛋白磷酸聚糖（PPG），其中一些被分泌，而另一些则在前鞭毛体和变形虫的表面被发现。这些蛋白质被认为在寄生虫的传播，入侵和随后的细胞内存活中很重要。 PPG的结构和功能在L. major和L. mexicana的情况下是物种和阶段特定的，但是迄今为止，尚无关于L. donovani的此类信息。这项研究首次通过共聚焦显微镜，流式细胞术和Western blot方法使用抗StoGluc酸钠（SSG）和来自比哈尔邦（印度）的多诺尼乳杆菌（L. donovani）的敏感临床分离株中的PPG进行了初步表征（定位） L.主要PPG。共聚焦显微镜分析显示，前鞭毛体和变形虫在其细胞膜和鞭毛袋膜上均具有PPG，但其表达在不同的分离物中是可变的。通过FACS和蛋白质印迹的定量分析表明，在抗SSG的分离物中PPG条带的表达和强度更高。这项研究表明，PPG参与耐药机制的可能性以及使用PPG丰度作为鉴定印度黑热病中耐药临床分离株的标志物的可能性。 2007剑桥大学出版社。

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