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首页> 外文期刊>Pakistan journal of zoology >Optimization of Fermentation Conditions for Enhanced Glutamic Acid Production by a Strain of Corynebacterium glutamicum NIAB BNS-14
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Optimization of Fermentation Conditions for Enhanced Glutamic Acid Production by a Strain of Corynebacterium glutamicum NIAB BNS-14

机译:谷氨酸棒杆菌NIAB BNS-14菌株提高谷氨酸生产发酵条件的优化

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摘要

Corynebacterium glutamicum NIAB BNS-14 was used for enhancing glutamic acid production for which eight different carbon (glucose, fructose, sucrose, maltose, lactose, dextrin, sorbose, and galactose) and seven nitrogen sources (ammonium chloride, ammonium sulphate, ammonium nitrate, ammonium phosphate, urca, potassium nitrate and sodium nitrate) alongwith various concentrations of biotin (0.5, 1.0, 2.5, 5.0, 10, 15 mu g/ml) were used. The strain gave maximum production as 8.5g/l of glutamic acid at 10% fructose and 3% ammonium nitrate with 10 mu g/ml biotin at 30 degrees C and 150rpm after 72hrs of fermentation. The cell mass obtained was 21.1g/l.
机译:谷氨酸棒杆菌NIAB BNS-14用于增强谷氨酸的生产,其中八个碳(葡萄糖,果糖,蔗糖,麦芽糖,乳糖,糊精,山梨糖和半乳糖)和七个氮源(氯化铵,硫酸铵,硝酸铵,使用磷酸铵,尿酸,硝酸钾和硝酸钠,以及各种浓度的生物素(0.5、1.0、2.5、5.0、10、15μg / ml)。发酵72小时后,菌株在30℃和150rpm下以10%果糖和3%硝酸铵与10μg/ ml生物素的最大产量为8.5g / l谷氨酸。获得的细胞质量为21.1g / l。

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