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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Microdissection of tissue sections: application to the molecular genetic characterisation of premalignant lesions.
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Microdissection of tissue sections: application to the molecular genetic characterisation of premalignant lesions.

机译：组织切片显微切割：应用于恶变前病变的分子遗传学表征。

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摘要

The characterisation of the early molecular genetic events of tumor development depends on the selective procurement of histopathologically defined small cell populations from premalignant tissue. In order to obtain high-quality DNA, mRNA and proteins from these small tissue samples and even from single cells, tissue microdissection is one of the most useful techniques, becoming increasingly important for molecular pathologists. Using different microdissection techniques which allow the isolation of morphologically defined cell populations under direct visualisation, it is now feasible to study molecular genetic events that drive the multistep evolution in tumours. This review aims to present the current techniques of tissue microdissection and these techniques are discussed in the light of their ability to isolate premalignant cell populations in particular. Furthermore, we describe the subsequent application of several multiplex molecular analyses for characterising the microdissected premalignant cells. Applying these advanced techniques, alterations in the cellular DNA or the fluctuation of expressed genes that correlate with a particular stage of carcinogenesis can ultimately be compared within or between individual patients. Thus, these new technologies will have an enormous impact on molecular pathology with several diagnostic, prognostic and therapeutic implications. Copyright 2000 S. Karger AG, Basel.
机译：肿瘤发展的早期分子遗传事件的表征取决于从恶变前组织选择性获取组织病理学定义的小细胞群。为了从这些小的组织样本甚至从单个细胞中获得高质量的DNA，mRNA和蛋白质，组织显微解剖是最有用的技术之一，对于分子病理学家而言变得越来越重要。使用不同的显微解剖技术，可以在直接观察下分离出形态学确定的细胞群，现在研究驱动肿瘤多步进化的分子遗传事件是可行的。这篇综述旨在介绍当前的组织显微解剖技术，并根据它们分离恶性前细胞群的能力来讨论这些技术。此外，我们描述了用于表征显微解剖的恶性前细胞的几种多重分子分析的后续应用。应用这些先进的技术，可以最终在个体患者之间或个体之间比较细胞DNA的改变或表达基因的波动与癌变的特定阶段相关。因此，这些新技术将对分子病理学产生巨大影响，并具有多种诊断，预后和治疗意义。版权所有2000 S. Karger AG，巴塞尔。

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