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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >DNA topoisomerase II-alpha immunoreactivity as a marker of tumor aggressiveness in invasive breast cancer.
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DNA topoisomerase II-alpha immunoreactivity as a marker of tumor aggressiveness in invasive breast cancer.

机译：DNA拓扑异构酶II-alpha免疫反应性作为浸润性乳腺癌中肿瘤侵袭性的标志物。

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OBJECTIVE: The nuclear enzyme DNA topoisomerase (topo) II breaks and rejoins DNA strands; its isoform topo IIalpha is associated with active cell proliferation of mammalian cells. The aim of this study was to examine the relationship between the expression of topo IIalpha and biological behavior markers in breast cancer. METHODS: Formalin-fixed, paraffin-embedded tissue from 88 samples of infiltrating breast cancer was immunohistochemically stained for topo IIalpha. For each case, a topo IIalpha index was determined by image analysis. Similar indexes were available for Ki-67 protein, a known cell proliferation marker, and p53, bcl-2 and c-erbB-2 oncoproteins. Each case had been staged and graded and the patients had been followed up for a mean period of 61.62 months. RESULTS: Elevated topo IIalpha immunopositivity (in >10% of malignant nuclei) was detected in 22 tumors, and this immunostatus was statistically associated with poor nuclear differentiation, absence of steroid hormone receptors, high Ki-67 immunoexpression, p53 protein accumulation and c-erbB-2 protein overexpression. Topo IIalpha expression was not linked with disease extent (stage or lymph node status). Neither proliferation marker (topo IIalpha or Ki-67) had any significant influence on the patients' recurrence-free survival. CONCLUSION: From the above results, we conclude that topo IIalpha overexpression appears to be linked with cellular dedifferentiation and potentially aggressive tumor phenotype in invasive breast cancer. Copyright 2001 S. Karger AG, Basel
机译：目的：核酶DNA拓扑异构酶（拓扑）II断裂并重新结合DNA链。其同工型topo IIalpha与哺乳动物细胞的活跃细胞增殖有关。这项研究的目的是检查乳腺癌中topo IIalpha的表达与生物学行为标志之间的关系。方法：对88例浸润性乳腺癌样本中福尔马林固定，石蜡包埋的组织进行免疫组织化学染色，以检测topo IIalpha。对于每种情况，都通过图像分析确定了topo IIalpha指数。对于已知的细胞增殖标志物Ki-67蛋白以及p53，bcl-2和c-erbB-2癌蛋白，也可获得类似的指标。每个病例都经过了分级和分级，并对患者进行了平均61.62个月的随访。结果：在22个肿瘤中检测到topo IIalpha免疫阳性（在大于10％的恶性核中），并且这种免疫状态与核分化差，类固醇激素受体缺失，高Ki-67免疫表达，p53蛋白积累和c- erbB-2蛋白过表达。 Topo IIalpha表达与疾病程度（阶段或淋巴结状态）无关。两种增殖标记物（拓扑IIalpha或Ki-67）均对患者的无复发生存率没有任何重大影响。结论：根据以上结果，我们得出结论，在侵袭性乳腺癌中，topo IIalpha的过度表达似乎与细胞去分化和潜在的侵袭性肿瘤表型有关。版权所有2001 S. Karger AG，巴塞尔

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