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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Oligoclonality of early lesions of the urothelium as determined by microdissection-supported genetic analysis.
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Oligoclonality of early lesions of the urothelium as determined by microdissection-supported genetic analysis.

机译：通过显微解剖支持的遗传分析确定尿路上皮早期病变的卵圆。

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AIM: To contribute to the ongoing discussion of clonality of human urothelial cancer it was considered a valuable approach to analyze multiple areas from cystectomy specimens for deletions of chromosomes known to be involved early in bladder cancer development. MATERIAL AND METHODS: Thus, in 86 biopsies of 4 human cystectomies with different histological findings (maximal diagnosis: pT1G2, pTaG3, pT2G2, normal) loss of heterozygosity (LOH) was investigated as a deletion marker using markers of chromosomes 8p, 9p, 9q and 17p. Findings were compared to histology of the lesion. RESULTS: Findings indicate: (1) no changes in the markers investigated in the bladder with histologically normal urothelium in contrast to detection of LOH in normal urothelium of tumour-bearing bladders; (2) an accumulation of the number of LOH with increasing malignancy of lesions within one bladder, and (3) indications of oligoclonal neoplastic lesions in two of the urinary bladders investigated. CONCLUSIONS: The investigation of multiple lesions within one bladder presents a snapshot of genetic changes in differently advanced tumour stages. The hypotheses of tumour evolution and oligoclonality as derived from our LOH data need to be supported by deletion-independent clonality studies as X-chromosomal inactivation analysis. Copyright 2001 S. Karger AG, Basel
机译：目的：为促进对人类尿路上皮癌克隆性的持续讨论，它被认为是一种分析膀胱切除术标本中多个区域的已知染色体缺失的有价值方法，该染色体已知参与了膀胱癌的早期发展。材料和方法：因此，在86例具有不同组织学发现（最大诊断：pT1G2，pTaG3，pT2G2，正常）的人膀胱切除术的活检中，使用了染色体8p，9p，9q的标记作为缺失标记研究了杂合性缺失和17便士。将发现与病变的组织学进行比较。结果：研究结果表明：（1）在组织学上正常的尿路上皮中，与正常的膀胱肿瘤尿路上皮中的LOH相比，在具有正常组织学特征的膀胱中所研究的标志物没有变化; （2）LOH数量的累积随着病变在一个膀胱内恶性程度的增加而增加，并且（3）在所研究的两个膀胱中有寡克隆肿瘤病变的迹象。结论：对一个膀胱内多个病变的研究提供了不同晚期肿瘤阶段遗传变化的快照。从我们的LOH数据得出的肿瘤进化和寡克隆性假设需要得到独立于缺失的克隆性研究（如X染色体失活分析）的支持。版权所有2001 S. Karger AG，巴塞尔

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