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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Expression of murine N-MYC by insertion of retrovirus sequences in a murine macrophage cell line (RAW264).
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Expression of murine N-MYC by insertion of retrovirus sequences in a murine macrophage cell line (RAW264).

机译：通过在鼠巨噬细胞系（RAW264）中插入逆转录病毒序列来表达鼠N-MYC。

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摘要

RAW264 cells, reported to be originated from Abelson-virus-induced B lymphomas, are widely used as a murine monocyte cell line. We found that RAW264 show enhanced expression of murine N-MYC. Murine cDNA clones associated with N-MYC were separated from (lambda)gt11 cDNA library constructed by using mRNA from the macrophage cell line, RAW264 cells. Sequencing analysis of the longest cDNA clone N-MYCL showed that the length of the coding region was 18 bases shorter than that of the predicted full length N-MYC cDNA, and the 3' untranslated region had the 5' long terminal repeat (LTR) sequence of the Moloney-like proviral sequence, suggesting the expression of N-MYC by insertion of the proviral sequence. This suggests that expression of N-MYC plays a role in the establishment of macrophage cell line RAW264. Integration of LTR and overexpression of the N-MYC gene might have existed in the parental lymphoma cells, playing a role in the development of lymphoma or in the establishment of macrophage cell line. Copyright 2003 S. Karger AG, Basel
机译：RAW264细胞据报道起源于Abelson病毒诱导的B淋巴瘤，被广泛用作鼠单核细胞系。我们发现RAW264显示出增强的鼠N-MYC表达。使用来自巨噬细胞系RAW264细胞的mRNA，从λgt11cDNA文库中分离出与N-MYC相关的鼠cDNA克隆。最长的cDNA克隆N-MYCL的测序分析表明，编码区的长度比预测的全长N-MYC cDNA的短18个碱基，并且3'非翻译区的5'长末端重复序列（LTR） Moloney样原病毒序列的序列，提示通过插入原病毒序列来表达N-MYC。这表明N-MYC的表达在巨噬细胞系RAW264的建立中起作用。 LTR的整合和N-MYC基因的过表达可能存在于亲代淋巴瘤细胞中，在淋巴瘤的发展或巨噬细胞系的建立中发挥作用。版权所有2003 S. Karger AG，巴塞尔

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