...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pest Management Science >Inheritance of resistance to Bacillus thuringiensis Cry1 Ac toxin in Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) from India
【24h】

Inheritance of resistance to Bacillus thuringiensis Cry1 Ac toxin in Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) from India

机译：印度棉铃虫（Hubner）（鳞翅目：夜蛾科）对苏云金芽孢杆菌Cry1 Ac毒素的抗性遗传。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: The cotton bollworm, Helicoverpa armigera (Hiibner), is one of the most serious insect pests of cotton. It has developed resistance to almost all groups of chemical insecticides because of their intensive use. The failure of insecticides to control H. armigera has been a strong incentive for the adoption of transgenic cotton (Bt cotton). However, the value of Bt could be diminished by widespread resistance development to Bt toxins in insect populations. Therefore, understanding the genetic basis of resistance is essential for developing and implementing strategies to delay and monitor resistance. RESULTS: A resistant strain designated as BM-R was obtained from the cross of adults from Bathinda and Muktsar , Punjab, India, which showed the highest survival (60.68%) and LC_(50) value (1.396 μg mL~(-1) diet). Similarly, a laboratory-maintained strain from Hoshiarpur, Punjab, showed maximum susceptibility to Cry1 Ac toxin with the lowest LC_(50) value (0.087 μg mL~(-1)), and was designated as HP-S. The genetic purity of both strains was confirmed by RAPD profile analysis at each generation, and genetic similarity reached more than 90% after the third generation. Continuous maintenance of the resistant BM-R strain on Cryl Ac resulted in an increase in LC_(50) from 0.531 μg mL~(-1) in F_0 to 4.28 μg mL~(-1) in F_(14) and 7.493 μg mL~(-1) in F_(19), while the LC_(50) values for HP-S larvae on diet without Cry1Ac increased to 0.106 and 0.104 μg mL~(-1), which lay within the fiducial limits of the baseline LC_(50) value. The mode of inheritance of resistance was elucidated through bioassay response of resistant, susceptible heterozygotes and backcross progeny to Cry1Ac incorporated in semi-synthetic diet. CONCLUSION: Based on dominance, degree of dominance and backcross values, resistance was inferred to be polygenic, autosomal and inherited as a recessive trait.
机译：背景：棉铃虫Helicoverpa armigera（Hiibner）是最严重的棉花害虫之一。由于其大量使用，它已对几乎所有类型的化学杀虫剂产生了抗药性。杀虫剂未能控制棉铃虫一直是采用转基因棉花（Bt棉花）的强烈动机。但是，由于昆虫种群中对Bt毒素的广泛耐药性发展，Bt的价值可能会降低。因此，了解抗药性的遗传基础对于制定和实施延迟和监测抗药性的策略至关重要。结果：从印度的旁遮普邦的Baathinda和Muktsar的成年人的杂交物中获得了一种名为BM-R的抗性菌株，其存活率最高（60.68％），LC_（50）值最高（1.396μgmL〜（-1））。饮食）。同样，来自旁遮普邦Hoshiarpur的实验室维护菌株显示出对Cry1 Ac毒素的最大敏感性，具有最低的LC_（50）值（0.087μgmL〜（-1）），并被指定为HP-S。这两个菌株的遗传纯度在每一代都通过RAPD谱分析得到了证实，并且在第三代之后遗传相似性达到了90％以上。 Cryl Ac上抗性BM-R菌株的持续维持导致LC_（50）从F_0中的0.531μgmL〜（-1）增加到F_（14）中的4.28μgmL〜（-1）和7.493μgmL F_（19）中的〜（-1），而不含Cry1Ac的日粮中HP-S幼虫的LC_（50）值增加至0.106和0.104μgmL〜（-1），处于基线LC_的基准范围内（50）值。通过对半合成饮食中掺入的Cry1Ac的抗药性，易感杂合子和回交子代进行生物测定反应，阐明了抗药性的遗传模式。结论：根据优势度，优势度和回交值，可以推断出抗性是多基因的，常染色体的并且是遗传的隐性性状。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号