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首页> 外文期刊>Pest Management Science >Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants
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Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants

机译：通过新开发的实时荧光定量PCR技术定量评估熏蒸土壤中的尖孢镰刀菌，以评估熏蒸剂对草莓植物枯萎病的功效

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摘要

BACKGROUND: Two soil fumigants, chloropicrin (CP) and dimethyl disulfide (DMDS), were used to control Fusarium wilt disease (FWO) which caused large economic losses in strawberries. The fumigants were evaluated alone and in combination in a laboratory study and in strawberry greenhouses. RESULTS: Laboratory tests found that combinations of CP and DMDS indicated a positive synergistic activity on Fusarium oxysporum. A newly developed quantitative assay for F. oxysporum involving real-time PCR was used successfully to evaluate F. oxysporum control by the fumigants; it provided similar results to the selective medium but was less time-consuming and less labor intensive. Greenhouse trials revealed that the combination of CP and DMDS successfully suppressed the incidence of FWD and sharply reduced the population density of F. oxysporum, which significantly increased fruit branch number and maintained a good strawberry yield, higher than methyl bromide (MB) treatment. All of the treatments provided significantly better results than the non-treated control. CONCLUSION: This study confirms that the newly developed real-time PCR quantitative assay for F. oxysporum was suitable for the control efficacy evaluation of soil fumigants and that the novel fumigant combination of CP and DMDS offers a promising effective alternative to MB for the control of F. oxysporum in strawberry greenhouses.
机译：背景：使用两种土壤熏蒸剂，即氯化苦（CP）和二甲基二硫（DMDS）来控制镰刀菌枯萎病（FWO），该病导致草莓的大量经济损失。在实验室研究和草莓温室中对熏蒸剂进行了单独评估或组合评估。结果：实验室测试发现CP和DMDS的组合表明对尖孢镰刀菌具有积极的协同活性。一种新开发的涉及实时荧光定量PCR的oxysporum定量测定方法已成功用于评估熏蒸剂对oxysporum的控制。它提供了与选择性培养基相似的结果，但耗时少且劳动强度低。温室试验表明，CP和DMDS的组合成功抑制了FWD的发生并显着降低了尖孢镰刀菌的种群密度，从而显着增加了果枝数并保持了较高的草莓产量，高于甲基溴（MB）处理。与未治疗的对照相比，所有治疗均提供了明显更好的结果。结论：这项研究证实，最新开发的实时荧光定量PCR技术可用于测定农杆孢菌的土壤熏蒸剂的防治效果，CP和DMDS的新型熏蒸剂组合为MB的控制提供了有效的替代方法。草莓温室的F. oxysporum。

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