• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pharmaceutical Biology >Genotoxic effects of Campomanesia xanthocarpa extracts on Allium cepa vegetal system
【24h】

Genotoxic effects of Campomanesia xanthocarpa extracts on Allium cepa vegetal system

机译：桔梗桔梗提取物对洋葱韭菜植物系统的遗传毒性作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Context: Campomanesia xanthocarpa Berg. (Myrtaceae), popularly known in Brazil as guabiroba, is a plant used as antidiarrheic, anti-inflammatory and antirheumatic agents, and in stomach and hepatic disorders. Objective: The antiproliferative and genotoxic effects of aqueous extracts and essential oil of C. xanthocarpa were evaluated. Materials and methods: Cytotoxicity and genotoxicity of the aqueous extracts (6 and 30 mg/mL) and essential oil (0.25%, v/v) obtained from leaves of C. xanthocarpa were evaluated using the Allium cepa L. (Amaryllidaceae) assay. Mitotic index was calculated as the percentage of dividing cells of total cells observed; chromosome abnormalities were observed and counted during cell division. Additionally, the composition of the essential oil and the quantification of the main compounds of the extracts were determined by gas chromatography/mass spectrometry and high performance liquid chromatography coupled with diode array detector, respectively. Results and discussion: Aqueous extracts (6 and 30 mg/mL) led to a reduction of 67.7% and 34.1% of the mitotic index, respectively, whereas the treatment with essential oil caused a 48.2% reduction in the mitotic index, when compared with negative control. Chromosomal mutations were observed and included anaphase bridges, delay chromosome, break chromosome, as well as metaphase with disorganized chromosomal and binuclear cells. The main compounds of the essential oil were p-caryophyllene (8.87%), viridiflorol (6.40%), spathulenol (5.16%), 5-cadinene (4.92%), linalool (4.46%) and oc-cadinol (4.25%). Gallic acid (3.19%), chlorogenic acid (1.04%), quercetin (2.97%) and rutin (4.82%) were identified in an aqueous extract (30 mg/mL). Conclusion: Our results demonstrated that genotoxic and antiproliferative activities are present in C. xanthocarpa infusions using the in vivo onion root-tip cell test.
机译：背景：Camponesnes xanthocarpa Berg。 （桃金娘科），在巴西俗称瓜比罗巴（Gubiroba），是用作止泻，消炎和抗风湿药以及胃和肝病的植物。目的：评价桔梗水提取物和香精油的抗增殖和遗传毒性作用。材料和方法：用葱属（Amaryllidaceae）测定法评估了从角果果叶获得的水提取物（6和30 mg / mL）和精油（0.25％，v / v）的细胞毒性和遗传毒性。计算有丝分裂指数为观察到的总细胞中分裂细胞的百分比;在细胞分裂期间观察并计数染色体异常。另外，分别通过气相色谱/质谱和高效液相色谱结合二极管阵列检测器确定精油的组成和提取物中主要化合物的定量。结果与讨论：水提取物（6和30 mg / mL）分别导致有丝分裂指数降低67.7％和34.1％，而精油处理与之相比使有丝分裂指数降低48.2％。阴性对照。观察到染色体突变，包括后期桥梁，延迟染色体，断裂染色体以及具有杂乱的染色体和双核细胞的中期。香精油的主要化合物是对叶石竹烯（8.87％），viridiflorol（6.40％），spathulenol（5.16％），5-cadinene（4.92％），linalool（4.46％）和oc-cadinol（4.25％）。在水提取物中（30 mg / mL）鉴定出没食子酸（3.19％），绿原酸（1.04％），槲皮素（2.97％）和芦丁（4.82％）。结论：我们的结果表明，使用体内洋葱根尖细胞试验，在果皮角果注射液中存在遗传毒性和抗增殖活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号