...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >RGD-grafted thermoreversible polymers to facilitate attachment of BMP-2 responsive C2C12 cells
【24h】

RGD-grafted thermoreversible polymers to facilitate attachment of BMP-2 responsive C2C12 cells

机译：RGD接枝的热可逆聚合物可促进BMP-2反应性C2C12细胞的附着

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study was to design thermoreversible biomaterials for enhanced adhesion of bone morphogenctic protein-2 (BMP-2)-responsive cells. Peptides containing the arginine-glycine-aspartic acid (RGD) sequence were conjugated to N-isopropylacrylamide (NiPAM) polymers via amine-reactive N-acryloxysuccinimide (NASI) groups. In monolayer cultures, the adhesion of BMP-2-responsive C2C12 cells to RGD-grafted NiPAM/NASI surfaces was significantly higher than adhesion on ungrafted NiPAM/NASI surfaces. Although the morphology of cells adhered to RGD-grafted NiPAM/NASI surfaces was comparable to cells adhered on tissue culture polystyrene (TCPS), long-term cell growth was limited on the NiPAM/NASI surfaces, even for RGD-grafted surfaces. Treatment of C2C12 cells with recombinant BMP-2 induced dose-dependent osteoblastic differentiation as assessed by alkaline phosphatase (ALP) activity. In the absence of BMP-2, cells cultured on NiPAM/NASI polymers (either grafted with RGD peptide or not) expressed significantly higher levels of ALP activity than the cells cultured on TCPS, indicating that the polymer surfaces induced some osteoblastic activity in C2C12 cells without the need for BMP-2. We conclude that NiPAM-based thermoreversible biomaterials, despite their limited ability to support cell growth, allowed an enhanced expression of the chosen osteogenic marker (ALP) by C2C12 cells in vitro. (c) 2005 Elsevier Ltd. All rights reserved.
机译：这项研究的目的是设计热可逆生物材料，以增强骨形态发生蛋白2（BMP-2）反应细胞的粘附。含有精氨酸-甘氨酸-天冬氨酸（RGD）序列的肽通过胺反应性N-丙烯酰氧基琥珀酰亚胺（NASI）基团与N-异丙基丙烯酰胺（NiPAM）聚合物偶联。在单层培养中，BMP-2反应性C2C12细胞对RGD移植的NiPAM / NASI表面的粘附力显着高于未移植的NiPAM / NASI表面的粘附力。尽管粘附在RGD接枝的NiPAM / NASI表面的细胞形态与粘附在组织培养聚苯乙烯（TCPS）上的细胞相当，但即使对于RGD接枝的表面，长期细胞生长仍在NiPAM / NASI表面受到限制。通过碱性磷酸酶（ALP）活性评估，用重组BMP-2处理C2C12细胞可诱导剂量依赖性成骨细胞分化。在没有BMP-2的情况下，在NiPAM / NASI聚合物上培养的细胞（无论是否移植有RGD肽）表达的ALP活性水平均显着高于TCPS上培养的细胞，表明该聚合物表面在C2C12细胞中诱导了某些成骨活性无需BMP-2。我们得出的结论是，尽管基于NiPAM的热可逆生物材料支持细胞生长的能力有限，但仍允许C2C12细胞在体外增强所选成骨标记（ALP）的表达。 （c）2005 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号