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首页> 外文期刊>Physiological Research >Temporal relationship between lens protein oxidation and cataract development in streptozotocin-induced diabetic rats
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Temporal relationship between lens protein oxidation and cataract development in streptozotocin-induced diabetic rats

机译：链脲佐菌素诱导的糖尿病大鼠晶状体蛋白氧化与白内障发展之间的时间关系

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We compared the progression of lens opacification with the time course of oxidation of lens proteins under conditions of streptozotocin-induced experimental diabetes in rats. By the end of the 17th week, approx. 50 % of the diabetic animals developed mature cataracts. During the following month, 95 % of the eyes in the diabetic group became cataractous. In the course of lens opacification we observed a time-dependent increase in the content of protein carbonyls and decrease in the concentration of protein sulfhydryls in the lenses of diabetic animals. Significantly higher protein carbonyl (p<0.01) and lower protein sulfhydryl (p<0.001) content was found in lenses with the advanced stage of cataract when compared with the diabetic lenses still transparent. We showed that the values of protein carbonyls exceeding 1.2 nmol/mg protein and of sulfhydryls falling below 60 nmol/mg protein corresponded to an approximately 50 % incidence of mature cataract development. At the end of the 34th week, when all lenses of diabetic rats became cataractous, the corresponding values of protein carbonyls and sulfhydryls were 2.5 nmol/mg protein and 27 nmol/mg protein, respectively. The main finding of this study is the disclosure of quantitative relationship between the degree of protein oxidation and the rate of advanced cataract development in the widely used model of streptozotocin-induced experimental diabetes in rats.
机译：我们比较了在链脲佐菌素诱导的大鼠实验性糖尿病条件下晶状体混浊的进展与晶状体蛋白氧化的时间过程。到第17周结束时，大约50％的糖尿病动物发展出成熟的白内障。在接下来的一个月中，糖尿病组中95％的眼睛患有白内障。在晶状体混浊的过程中，我们观察到糖尿病动物晶状体中蛋白质羰基的含量随时间的增加和蛋白质巯基浓度的降低。与仍透明的糖尿病性晶状体相比，在患有白内障晚期的晶状体中发现明显较高的蛋白质羰基（p <0.01）和较低的蛋白质巯基（p <0.001）含量。我们表明，超过1.2 nmol / mg蛋白质的羰基蛋白和低于60 nmol / mg蛋白质的巯基值对应大约50％的成熟白内障发生率。在第34周结束时，当糖尿病大鼠的所有晶状体都变成白内障时，相应的蛋白质羰基和巯基蛋白值分别为2.5 nmol / mg蛋白和27 nmol / mg蛋白。这项研究的主要发现是，在广泛使用的链脲佐菌素诱发的大鼠实验性糖尿病模型中，揭示了蛋白质氧化程度与晚期白内障发生率之间的定量关系。

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