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Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed

机译：基于适配体的麦角生物碱从麦角污染的黑麦饲料中提取

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摘要

Ergot alkaloids are mycotoxins which can be found in food based on cereal-crops, due to a contamination of plants by fungi of the genus Claviceps. The ingestion of ergot contaminated cereal crops can lead to a severe poisoning known as ergotism. For food and feed safety purposes, the extraction of ergot alkaloids from ergot contaminated flour was investigated. For the specific recognition of ergot alkaloids, DNA aptamer ligands specially selected for ergot alkaloids were grafted onto silica gel in order to construct a specific solid phase extraction system. The aptamer-functionalized silica gels were used to extract ergot alkaloids from a contaminated rye feed sample. The presence of ergot alkaloids eluted from the aptamer-functionalized silica gels was analyzed using LC-QTOF-MS. By using this simple system, it was possible to specifically extract ergosine, ergokryptine and ergocornine from an ergot contaminated rye feed sample. This aptamer-based extraction tool shows the applicability of aptamers for the specific extraction of toxins or natural compounds from turbid matrices in a one-step procedure.
机译：麦角生物碱是霉菌毒素，由于谷氨酸类杆菌对植物的污染，可以在谷物作物中发现。摄入麦角污染的谷物作物会导致严重的中毒，称为麦角症。为了食品和饲料安全，研究了从麦角污染的面粉中提取麦角生物碱。为了特异性识别麦角生物碱，将专门选择用于麦角生物碱的DNA适体配体接枝到硅胶上，以构建特定的固相萃取系统。适体官能化的硅胶用于从受污染的黑麦饲料样品中提取麦角生物碱。使用LC-QTOF-MS分析了从适体官能化硅胶上洗脱的麦角生物碱的存在。通过使用这个简单的系统，可以从受麦角污染的黑麦饲料样品中专门提取出麦芽碱，麦角隐亭和麦角碱。这种基于适体的提取工具显示了适体的适用性，可通过一步步骤从混浊的基质中特异性提取毒素或天然化合物。

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