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Mutant sialidase having trans-sialidase activity for use in production of sialylated glycans

机译:具有反唾液酸酶活性的突变唾液酸酶,用于生产唾液酸化的聚糖

摘要

The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo-saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.
机译:本发明提供了一种具有反唾液酸酶活性的突变酶(EC 3.2.1.18),其特征在于与它的亲本唾液酸酶相比,反式唾液酸酶:唾液酸酶的比例提高。此外,该酶可以用于单唾液酸和寡糖的唾液酸化的方法,包括半乳糖-低聚糖(GOS),果糖-低聚糖(FOS),麦芽-低聚糖(MOS),异麦芽-低聚糖(IMO),乳果糖,蜜力比糖,麦芽糖,糖基蔗糖,乳糖蔗糖和岩藻糖。用突变酶生产的反唾液酸单糖和寡糖可用于制备婴儿配方食品,益生元营养补品和食品补品。

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