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detection of mycotoxins in the nanotechnology platform. in particular, ochratoxin a

机译:在纳米技术平台中检测真菌毒素。特别是曲霉毒素a

摘要

the present invention relates to, in a broad sense, the art of the development and design of a highly sensitive and selective electrochemical biosensor for the detection of mycotoxins.the invention belongs to the field of nanotechnology for the development of tools for the analysis of biochemical and electrochemical bics (concerning, in particular, a process permettanto detect quantitatively the ochratoxin a (ota) is a mycotoxin produced by several fungi (penicillium and aspergillus genera).the simplicity of the concept, the miniature size, low cost and ease of use are the basis for the marketing of such a product.in this context, the biosensor has been developed based on a simple strategy where a printed carbon electrode was modified with gold nanoparticles electrochemically by mercaptoethanol.a strand dna with high affinity for complexation of ota (anti ota aptamer) and a derivative ferrocénique noted, the fsc.the electrochemical studies, we were able to demonstrate the linearity of the response over the concentration range of interest, a longer shelf life.the selectivity for the analyte in question, and the ability to exclude the interference of bioactive substances.the analysis platform with a wide dynamic range (0.5 to 70 ng·ml (1) and a detection limit of 0.oil ng·ml (within the limits set by the european commission for agricultural products).this detection system was used for the quantification of ota in complex environments, such as roasted coffee beans and wine without any pretreatment of the samples.
机译:广义上,本发明涉及用于检测霉菌毒素的高灵敏度和选择性的电化学生物传感器的开发和设计领域。本发明属于用于生物化学分析工具开发的纳米技术领域和电化学bics(特别是关于permettanto定量检测the曲霉毒素a(ota)是几种真菌(青霉属和曲霉属)产生的霉菌毒素的方法。该概念的简单性,微型化,低成本和易用性在这种情况下,生物传感器是基于一种简单的策略开发的,其中印刷的碳电极通过巯基乙醇用金纳米颗粒进行电化学修饰。对ota络合具有高度亲和力的链dna(反ota aptamer)和衍生的ferrocénique指出,通过fsc。电化学研究,我们能够证明在目标浓度范围内的响应,更长的保存期限,所分析物的选择性以及排除生物活性物质干扰的能力。宽动态范围(0.5至70 ng·ml(1 )的检出限为0.oil ng·ml(在欧洲农产品委员会设定的检出限内)。此检出系统用于定量分析复杂环境中的ota,而无需进行任何预处理,例如烘焙过的咖啡豆和葡萄酒样本。

著录项

  • 公开/公告号TN2017000386A1

    专利类型

  • 公开/公告日2019-01-16

    原文格式PDF

  • 申请/专利权人 FACULTÉ DES SCIENCES DE TUNIS;

    申请/专利号TN20170000386

  • 发明设计人 NOUREDDINE RAOUAFI;WICEM ARGOUBI;

    申请日2017-09-07

  • 分类号C12Q1/68;G01N33/48;

  • 国家 TN

  • 入库时间 2022-08-21 12:00:42

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