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BARLEY WITH LOW HORDEIN CONTENT

机译：大麦醇溶蛋白含量低的大麦

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FIELD: food industry.;SUBSTANCE: invention refers to the field of biotechnology and food industry. Presented is a barley plant that yields grain and is homozygotic in at least two loci for the genetic variations having been bred, representing: a) allele wherein most of or all the genes coding B-hordein in Locus Hor2 are removed, and b) mutant allele in the barley Locus Lys3 so that the grain contains neither B-, nor C- hordeins, the said genetic variations present in Lines Riso 56 and Riso 1508 barley accordingly; absence of B-hordeins is to be revealed by absence of amplified DNA using primers: 5'B1hor: 5'-CAACAATGAAGACCTTCCTC-3', 3'B1hor: 5'-TCGCAGGATCCTGTACAACG-3', while absence of C-hordeins is to be revealed by absence of the 70 kDa strip during study of the grain alcohol-soluble extract by means of SDS-PAGE. Additionally presented are: barley grain cropped from the said plant; B- and C-hordein-free products produced from the said grain such as flour, malt and beer. Additionally described are methods for production of food products barley (flour, whole-grain flour, starch, malt) and beverages using grain cropped from the barley plant having the above characteristics. Proposed is a method for identification of barley grain suitable for production of a malt-based food product and/or beverage suitable for consumption by a person suffering from gluten-sensitive enteropathy which method includes: a) production of one or more materials: i) sample of a plant capable to yield the said grain, ii) grain, iii) malt produced from the grain, and/or iv) extract of the said grain; b) analysis of Stage a) material for presence of at least one hordein and/or at least one hordein-coding gene with selection of grain having the gene pattern of the above plant.;EFFECT: invention allows to manufacture B- and C-hordein-free malt-based food products or beverages.;27 cl, 14 dwg, 10 tbl, 10 ex
机译：领域：食品工业。;物质：发明是指生物技术和食品工业领域。呈现的是一种大麦植物，其产生谷粒并且在至少两个基因座上是纯合的，用于育种的遗传变异，代表：a）等位基因，其中在基因座Hor2中大部分或所有编码B-大麦醇溶蛋白的基因被去除，和b）突变大麦基因座Lys3中的等位基因，因此谷物既不含B-大麦醇溶蛋白，也不含C-大麦醇溶蛋白，相应的遗传变异相应地存在于品系Riso 56和Riso 1508中。使用引物：5'B1hor：5'-CAACAATGAAGACCTTCCTC-3'，3'B1hor：5'-TCGCAGGATCCTGTACAACG-3'来显示B-hordeins的缺失，而C-hordeins的缺失是通过SDS-PAGE研究可溶于谷物的醇溶性提取物时发现70 kDa条带缺失。还提供了：从所述植物中收获的大麦籽粒;由上述谷物生产的不含B和C大麦醇溶蛋白的产品，例如面粉，麦芽和啤酒。另外描述了使用从具有上述特征的大麦植物中收获的谷物生产食品大麦（面粉，全麦粉，淀粉，麦芽）和饮料的方法。提出了一种鉴定大麦籽粒的方法，该大麦籽粒适合生产适合麦麸敏感性肠病的人食用的基于麦芽的食品和/或饮料，该方法包括：a）生产一种或多种材料：i）能够产生所述谷粒的植物的样品，ii）谷粒，iii）由谷粒产生的麦芽，和/或iv）所述谷粒的提取物; b）分析阶段a）材料中是否存在至少一种大麦醇溶蛋白和/或至少一种大麦醇溶蛋白编码基因，并选择具有上述植物的基因模式的谷物;效果：本发明允许制造B-和C-不含大麦醇溶蛋白的麦芽食品或饮料。; 27 cl，14 dwg，10 tbl，10 ex

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