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Laser induced fluorescence detection in modern liquid chromatography with conventional and micro columns

机译:常规和微型色谱柱在现代液相色谱中的激光诱导荧光检测

摘要

In a method and a device for laser induced fluorescence detection in liquid chromatography a light beam (1) is directed from a laser source (2) towards a liquid flowing in a chosen flow direction for the detection of the presence of one or several substances. At a flow point illuminated by the light beam (1) the liquid is brought to emit a fluorescence radiation (3) which is received by a receiving unit (6). The light beam (1) is directed substantially perpendicularly to the flow direction of the liquid. The liquid is brought to pass the illuminated flow point in the form of a column with an accurately defined surface. The fluorescence radiation (3) is received in a direction which deviates from directions emanating from the illuminated flow point perpendicularly from the flow direction of the liquid column.
机译:在液相色谱法中用于激光诱导的荧光检测的方法和设备中,光束(1)从激光源(2)朝着沿选定流动方向流动的液体引导,以检测一种或多种物质的存在。在光束(1)照射的流动点,使液体发出荧光辐射(3),该荧光辐射被接收单元(6)接收。光束(1)基本垂直于液体的流动方向。使液体以具有精确限定的表面的柱的形式通过照明的流动点。在垂直于液柱的流动方向的方向上偏离荧光照射点的方向接收荧光辐射(3)。

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