摘要：
Objective To compare the cross-reactivity of four commercialized IgG ELISA kits for dengue virus,Zika virus,Japanese encephalitis virus and West Nile virus,and to evaluate the commercialized IgG kits for the flaviviruses by using serum samples from healthy people.Methods Convalescent serum samples from 3 cases each of dengue virus,Zika virus and Japanese encephalitis virus infections were selected to evaluate the cross-reactivity of the commercialized IgG ELISA kits by detecting IgG against the four viruses.The serum samples were collected from healthy people in Jiangmen where imported Zika cases were confirmed before collection,and four flavivirus IgG antibodies were detected.Finally,plaque reduction neutralization test was conducted to verify the positive result of Zika virus IgG antibody detected by ELISA.Results The test results of dengue fever,Zika and Japanese encephalitis in convalescent serum samples showed cross-reactions among dengue virus,Zika virus and Japanese encephalitis virus with the commercialized IgG kits.Among the 78 serum samples from healthy people,the positive rates of dengue,Zika,Japanese encephalitis and West Nile viruses were 0％,10.25％,47.43％ and 0％,respectively by ELISA.Further test showed that there was no neutralizing antibody in the 8 samples that were positive of IgG against Zika virus.Conclusions Cross reactivity was found in the commercialized flavivirus IgG ELISA kits,false positive results will be detected.It is recommended that ELISA test should be combined with other serological methods for clinical diagnosis and epidemiological investigation.%目的 比较登革病毒、寨卡病毒、日本脑炎病毒和西尼罗病毒四种商品化黄病毒IgG抗体检测试剂盒的交叉反应情况,并用健康人血清对商品化黄病毒IgG抗体检测试剂盒进行效果评价.方法 登革病毒、寨卡病毒和日本脑炎病毒感染病例(各3例)的恢复期血清,采用ELISA方法检测四种黄病毒IgG抗体,评估商品化黄病毒IgG抗体检测试剂盒的交叉反应情况,从此前出现过输入性寨卡病毒感染确诊病例的江门市采集健康人群血清,检测上述四种黄病毒IgG抗体,最后通过蚀斑减少中和实验验证ELISA方法检测的寨卡病毒IgG抗体阳性结果.结果 通过确诊登革热、寨卡和乙脑病例恢复期血清检测发现,已有商品化试剂盒在检测登革病毒、寨卡病毒和日本脑炎病毒阳性血清时存在交叉反应.IgG抗体ELISA检测试剂盒检测发现,78例健康人血清样本中登革病毒抗体阳性样本数为0例;寨卡病毒抗体阳性样本数为8例(阳性率10.25％);日本脑炎病毒抗体阳性样本数37例(阳性率47.43％);33例健康人血清样本中西尼罗病毒抗体阳性样本数为0例.蚀斑减少中和实验结果显示,8例寨卡病毒IgG抗体阳性样本均未检测到中和抗体.结论 商品化黄病毒IgG抗体ELISA检测试剂盒存在交叉反应,单纯运用ELISA试剂盒检测会出现假阳性结果,因此在进行血清学方法检测时应联合其他检测方法.
