Cytogenetic Analysis of American Chestnut (Castanea dentata) Using Fluorescent In Situ Hybridization

机译：利用荧光原位杂交技术对美国板栗（Castanea dentata）进行细胞遗传学分析

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The American chestnut (Castanea dentata), once known as 'The King of the Forest' in the Appalachian Mountains of the eastern United States and southeastern Canada, has been all but extirpated by chestnut blight disease caused by an Asiatic bark fungus, Cryphonectria parasitica. A group of scientists at The American Chestnut Foundation has been working since 1983 to develop blight resistant American chestnut by inter-species backcross breeding with the relatively resistant Chinese chestnut (Castanea mollissima). In an effort to facilitate this breeding program we have initiated cytogenetic studies of these two species and their F1 and backcross hybrids. Here we report our successes in preparing somatic chromosomes using enzymatic digestion of American chestnut seedling root tips and demonstrate the quality of the chromosome spreads using fluorescent in situ hybridization with ribosomal DNA probes. Our results show two 18S-28S rDNA sites, one major and one minor, and one 5S rDNA site in American chestnut. This is the first such report for American chestnut and our preliminary results appear similar for Chinese chestnut.

机译：美洲栗（Castanea dentata），曾经在美国东部和加拿大东南部的阿巴拉契亚山脉被称为“森林之王”，但由于亚洲树皮真菌Cryphonectria parasitica引起的栗枯病已几乎灭绝。美国栗子基金会的一组科学家自1983年以来一直在工作，通过种间回交育种和相对抗性的中国板栗（Castanea mollissima）开发抗白叶枯的美国板栗。为了促进该育种程序，我们已开始对这两个物种及其F1和回交杂种进行细胞遗传学研究。在这里，我们报告了我们利用美洲板栗幼苗根尖的酶消化方法制备体细胞染色体的成功，并证明了利用核糖体DNA探针进行荧光原位杂交的结果表明了染色体铺展的质量。我们的结果显示了美洲板栗中两个18S-28S rDNA位点，一个主要和一个未成年人，以及一个5S rDNA位置。这是美国栗的首份此类报告，我们的初步结果与中国栗相似。

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来源
《第四届国际板栗大会（Proceedings of Fourth International Chestnut Symposium)》|2008年|207-210|共4页
会议地点 Beijing(CN)
作者
N.Islam-Faridi; C.D.Nelson; P.H.Sisco; T.L.Kubisiak; F.V.Hebard; R.L.Paris; R.L.Phillips;
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作者单位

U.S.Forest Service,Southern Institute of Forest Genetics,Forest Tree Molecular Cytogenetics Laboratory,Department of Ecosystem Science and Management,Texas AM University,College Station TX 77843,USA;

U.S.Forest Service,Southern Institute of Forest Genetics,Harrison Experimental Forest,23332 Old Mississippi 67,Saucier,MS 39574,USA;

Headquarters Office,The American Chestnut Foundation,160 Zillicoa Street,Suite D,Asheville,NC 28801,USA;

U.S.Forest Service,Southern Institute of Forest Genetics,Harrison Experimental Forest,23332 Old Mississippi 67,Saucier,MS 39574,USA;

Meadowview Research Farm,The American Chestnut Foundation,14005 Glenbrook Avenue,Meadowview,VA 24361,USA;

Meadowview Research Farm,The American Chestnut Foundation,14005 Glenbrook Avenue,Meadowview,VA 24361,USA;

Department of Agronomy;

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正文语种 eng
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关键词
ribosomal DNA; 18S-28S rDNA; 5S rDNA; FISH; Chinese chestnut; backcross breeding;

机译：核糖体DNA； 18S-28S rDNA； 5S rDNA； FISH；板栗；回交育种;

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专利

1. Estimation of Total Phenolic Compounds and Non-Targeted Volatile Metabolomics in Leaf Tissues of American Chestnut (Castanea dentata), Chinese Chestnut (Castanea mollissima) and the Backcross Breeding Generations [J] . Jinyan She, Chathuri U. G. Mohottige, Mary King, 农业化学和环境（英文） . 2021,第002期
2. Cytogenetic analysis of American chestnut ( Castanea dentate ) using fluorescent in situ hybridization. [J] . Islam-Faridi N., Nelson C. D., Sisco P. H., Acta Horticulturae . 2009,第844期

机译：利用荧光原位杂交技术对美国板栗（ Castanea dentate ）进行细胞遗传学分析。
3. Comparison of the transcriptomes of American chestnut ( Castanea dentata ) and Chinese chestnut ( Castanea mollissima ) in response to the chestnut blight infection [J] . Abdelali Barakat, Denis S DiLoreto, Yi Zhang, BMC Plant Biology . 2009,第1期

机译：美国板栗（Castanea dentata）和中国板栗（Castanea mollissima）响应栗疫病的转录组比较。
4. Recovery of American chestnut characteristics following hybridization and backcross breeding to restore blight-ravaged Castanea dentata [J] . Matthew Diskin, Kim C. Steiner, Frederick V. Hebard Forest Ecology and Management . 2006,第1a3期

机译：杂交和回交育种后恢复美洲light板栗的恢复，恢复美洲板栗特性
5. Cytogenetic Analysis of American Chestnut (Castanea dentata) Using Fluorescent In Situ Hybridization [C] . International chestnut symposium . 2009

机译：美国栗子（Castanea Dentata）使用荧光杂交的细胞遗传学分析
6. Influence of mycoviruses on the population structure of the chestnut blight pathogen, Cryphonectria parasitica and recovery of American chestnut, Castanea dentata. [D] . Springer, Joshua Charles. 2013

机译：霉菌病毒对板栗疫病病原体寄生小ry（Cryphonectria parasitica）的种群结构以及美国板栗（Castanea dentata）的恢复。
7. Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection [O] . Abdelali Barakat, Denis S DiLoreto, Yi Zhang, 2009

机译：板栗枯萎病感染后美国板栗（Castanea dentata）和中国板栗（Castanea mollissima）的转录组比较
8. Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection [O] . Wheeler Nicholas, Powell William A, Baier Kathleen, 2009

机译：板栗枯萎病感染后美国板栗（ Castanea dentata ）和中国板栗（ Castanea mollissima ）的转录组比较
9. Molecular cytogenetics using fluorescence in situ hybridization. [R] . Gray, J. W., Kuo, W. L., Lucas, J., 1990

机译：使用荧光原位杂交的分子细胞遗传学。

Cytogenetic Analysis of American Chestnut (Castanea dentata) Using Fluorescent In Situ Hybridization

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