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首页> 外文会议>Colloidal Quantum Dots for Biomedical Applications; Progress in Biomedical Optics and Imaging; vol.7 no.19 >In vivo cell imaging with semiconductor quantum dots and noble-metal nanodots
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In vivo cell imaging with semiconductor quantum dots and noble-metal nanodots

机译：半导体量子点和贵金属纳米点的体内细胞成像

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摘要

Innovations in fluorescence microscopy of live cells involving new reagents and techniques reveal dynamic processes that were not previously observable and therefore unknown. Water soluble, bio-functionalized semiconductor quantum dots (QDs) provide advantages of much greater photostability compared to conventional fluorescent dyes, and, as a consequence, single QDs can be easily detected. QDs coupled to growth factor ligands behave similarly as the natural ligand and serve as highly fluorescent probes of the erbB family of tyrosine kinase receptors in living cells. Continuous confocal laser scanning microscopy and flow cytometry measurements of QDs combined with visible fluorescent fusions of the receptors have elucidated individual steps in the signaling cascades initiated by these receptors. This report highlights advantages and some disadvantages of QDs, such as size and blinking behavior that complicate some live cell imaging applications. The new class of noble metal nanodots constitute an attractive alternative to QDs in that they are not only highly fluorescent and photostable, but also, much smaller and non-toxic. We present a new synthesis method for the production of Au nanodots. We demonstrate that electrochemical synthesis allows the reproducible control of cluster size. The resulting clusters are more monodisperse than those formed by other methods and are stable over many months. We report their characterization using MALDI-TOF mass spectrometry and UV-VIS spectroscopy.
机译：活细胞荧光显微镜的创新涉及新的试剂和技术，揭示了以前观察不到的动态过程，因此是未知的。与常规荧光染料相比，水溶性，生物功能化的半导体量子点（QD）具有光稳定性更高的优点，因此，可以轻松检测单个QD。与生长因子配体偶联的QD的行为与天然配体相似，并充当活细胞中酪氨酸激酶受体erbB家族的高荧光探针。 QD的连续共聚焦激光扫描显微镜和流式细胞术测量与受体的可见荧光融合相结合，阐明了这些受体引发的信号级联反应中的各个步骤。该报告重点介绍了量子点的优缺点，例如大小和眨眼行为，使某些活细胞成像应用变得复杂。新型的贵金属纳米点构成了量子点的诱人替代品，因为它们不仅具有高荧光性和光稳定性，而且体积小且无毒。我们提出了一种新的生产金纳米点的合成方法。我们证明了电化学合成可以重现簇大小的控制。所得簇比通过其他方法形成的簇更单分散，并且在多个月内稳定。我们报告使用MALDI-TOF质谱和UV-VIS光谱进行表征。

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