Imaging Photosensitizer Distribution and Pharmacology using Multiphoton Microscopy

机译：使用多光子显微镜成像光敏剂分布和药理学

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Multiphoton microscopy is a powerful tool for imaging sub-cellular distribution of luminescent compounds present in living cells. We have used this tool to study the distribution and pharmacology of photosensitizers in tissue and tissue culture. Murine hepatoma tumor cells dosed with a photosensitizer were briefly photoactivated, then imaged for periods up to several hours. Using the photosensitizer Rose Bengal with green light activation, nearly immediate photolytic release of lysosomal enzymes resulted in catastrophic cell destruction within 5-30 minutes. The magnitude and rapidity of this response is markedly different than that observed with other photosensitizer agents, and is consistent with in vivo studies illustrating that Rose Bengal is capable of causing extremely rapid destruction of treated tumors.

机译：多光子显微镜是一种用于对活细胞中存在的发光化合物进行亚细胞分布成像的强大工具。我们已经使用此工具来研究光敏剂在组织和组织培养物中的分布和药理作用。用光敏剂对小鼠肝癌肿瘤细胞进行短暂的光活化，然后成像长达数小时。使用具有绿光活化作用的光敏剂玫瑰红，溶酶体酶几乎立即光解性释放，导致5-30分钟内破坏性细胞。这种反应的幅度和速度与其他光敏剂所观察到的速度和速度明显不同，并且与体内研究一致，该研究表明玫瑰孟加拉能够引起极快地破坏治疗的肿瘤。

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来源
《Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA》|2002年|p.112-118|共7页
会议地点 San Jose CA(US)
作者
Eric Wachter; Craig Dees; Jay Harkins; Walt Fisher; Tim Scott;
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作者单位

Photogen, Inc., Knoxville, TN 37931 USA;

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会议组织
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
microscopy; imaging; multiphoton; MPE; femtosecond; photosensitizer;

机译：显微镜成像多光子MPE；飞秒光敏剂;

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1. Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells [J] . A. Deniset-Besseau, S. Leveque-Fort, M. P. Fontaine-Aupart, Applied Optics . 2007,第33期

机译：多焦点多光子显微镜的三维时间分辨荧光成像在活细胞中的光敏剂研究
2. Combined non-linear laser imaging (two-photon excitation fluorescence microscopy, fluorescence lifetime imaging microscopy, multispectral multiphoton microscopy) in cutaneous tumours: first experiences. [J] . De-Giorgi V, Massi D, Sestini S, Journal of the European Academy of Dermatology and Venereology: JEADV . 2009,第3期

机译：皮肤肿瘤中的组合非线性激光成像（双光子激发荧光显微镜，荧光寿命成像显微镜，多光谱多光子显微镜）：首次体验。
3. Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo [J] . Camilla A. Thorling Yuri Dancik Clinton W. Hupple Gregory Medley Xin Liu Andrei V. Zvyagin Tom A. Robertson Frank J. Burczynski and Michael S. Roberts journal of biomedical optics . 2011,第8期

机译：多光子显微镜和荧光寿命成像为研究大鼠肝脏体内药物分布和代谢提供了一种新方法
4. Functional Imaging of Photosensitizers using Multiphoton Microscopy [C] . Eric Wachter, Craig Dees, Jay Harkins, Conference on Multiphoton Microscopy in the Biomedical Sciences Ⅱ Jan 20-22, 2002 San Jose, USA . 2002

机译：使用多光子显微镜对光敏剂进行功能成像
5. Examination of diagnostic features in multiphoton microscopy and optical coherence tomography images of ovarian tumorigenesis in a mouse model. [D] . Watson, Jennifer M. 2013

机译：在小鼠模型中检查卵巢肿瘤发生的多光子显微镜和光学相干断层扫描图像中的诊断特征。
6. Image segmentation for integrated multiphoton microscopy and reflectance confocal microscopy imaging of human skin in vivo [O] . Guannan Chen, Harvey Lui, Haishan Zeng 2015

机译：集成的多光子显微镜和人体皮肤反射共聚焦显微镜成像的图像分割
7. Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells [O] . A. Deniset-Besseau, S. Lévêque-Fort, M. P. Fontaine-Aupart, 2007

机译：多灶性多光子显微镜对活细胞的光敏剂研究的三维时间分辨荧光成像

Imaging Photosensitizer Distribution and Pharmacology using Multiphoton Microscopy

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