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首页> 外文会议>Energy-based treatment of tissue and assessment VIII >Cells Exposed to Nanosecond Electrical Pulses Exhibit Genetic Biomarkers of Mechanical Stress
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Cells Exposed to Nanosecond Electrical Pulses Exhibit Genetic Biomarkers of Mechanical Stress

机译：暴露于纳秒电脉冲的细胞表现出机械应力的遗传生物标记

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摘要

Exposures of cells to very short (＜1 μs) electric pulses in the megavolt/meter range have been shown to cause disruption of the plasma membrane. This disruption is often characterized by the formation of numerous small pores (＜2 nm in diameter) in the plasma membrane that last for several minutes, allowing the flow of ions into the cell. These small pores are called nanopores and the resulting damage to the plasma membrane is referred to as nanoporation. Nanosecond electrical pulse (nsEP) exposure can impart many different stressors on a cell, including electrical, electro-chemical, and mechanical stress. Thus, nsEP exposure is not a "clean" insult, making determination of the mechanism of nanoporation quite difficult. We hypothesize that nsEP exposure creates acoustic shock waves capable of causing nanoporation. Microarray analysis of primary adult human dermal fibroblasts (HDFa) exposed to nsEP, indicated several genes associated with mechanical stress were selectively upregulated 4 h post exposure. The idea that nanoporation is caused by external mechanical force from acoustic shock waves has, to our knowledge, not been investigated. This work will critically challenge the existing paradigm that nanoporation is caused solely by an electric-field driven event and could provide the basis for a plausible explanation for electroporation.
机译：已经证明细胞暴露于兆伏/米范围内的非常短的（＜1μs）电脉冲会导致质膜破裂。这种破坏的特征通常是在质膜上形成许多小孔（直径小于2 nm），这些小孔持续数分钟，从而使离子流进入细胞。这些小孔称为纳米孔，对质膜造成的损坏称为纳米孔。纳秒电脉冲（nsEP）暴露可在细胞上施加许多不同的压力源，包括电，电化学和机械应力。因此，nsEP暴露不是“干净”的侮辱，因此很难确定纳米穿孔的机理。我们假设nsEP暴露会产生能够引起纳米穿孔的声波。对暴露于nsEP的成年人类真皮成纤维细胞（HDFa）进行的微阵列分析表明，与机械应激相关的几个基因在暴露后4 h被选择性上调。就我们所知，尚未研究纳米穿孔是由声波的外部机械力引起的。这项工作将严重挑战现有的范式，即纳米穿孔仅由电场驱动的事件引起，并且可以为对电穿孔的合理解释提供基础。

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    Caleb C. Roth; Ronald A. Barnes Jr.; Bennett L. Ibey; Hope T. Beier; Erick K. Moen; Randolph D. Glickman;

  • 作者单位

    School of Medicine, Dept. of Radiological Sciences, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas, USA 78229;

    Dept. of Electrical Engineering, University of Texas San Antonio, 1 UTSA Circle, San Antonio, Texas, USA 78249;

    Radio Frequency Bioeffects Branch, Bioeffects Division, Human Effectiveness Directorate, 711th Human Performance Wing, Air Force Research Laboratory, 4141 Petroleum Road, JBSA Fort Sam Houston, Texas, USA 78234;

    Optical Radiation Bioeffects Branch, Bioeffects Division, Human Effectiveness Directorate, 711th Human Performance Wing, Air Force Research Laboratory, 4141 Petroleum Road, JBSA Fort Sam Houston, Texas, USA 78234;

    Dept. of Electrical Engineering - Electrophysics, University of Southern California, 920 Bloom Walk, SSC 502, Los Angeles, California, USA;

    Dept. of Ophthalmology, School of Medicine, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas, USA 78229;

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