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首页> 外文会议>Annual Meeting of the Japanese Association for Animal Cell Technology >THE X-LINK INHIBITOR OF APOPTOSIS PROTEIN (XIAP) ENHANCES THE SURVIVABILITY OF C2E7 HYBRIDOMA CELLS UNDER A SERUM DEPRIVED CONDITION
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THE X-LINK INHIBITOR OF APOPTOSIS PROTEIN (XIAP) ENHANCES THE SURVIVABILITY OF C2E7 HYBRIDOMA CELLS UNDER A SERUM DEPRIVED CONDITION

机译：凋亡蛋白（XIAP）的X链路抑制剂增强了C2E7杂交瘤细胞在血清剥夺条件下的活力

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Hybridoma C_2E_7 cell cultured in a serum deprived condition was only survived not longer than 5 days in a batch culture, whereas for the culture supplemented with 10% of serum showed a far superior proliferation and survivability. The serum supplemented culture was able to survive for more than 9 days in a batch culture and recorded a 6.6 fold increase in maximum cell density. After 5 days of batch culture, the viability of the control culture still more than 50% viable, while for the serum deprivedculture the viability of the cells dropped to 17%. The mechanism of the cell death is predominantly via apoptosis which is shown in a classical ladder type pattern on a DNA gel and florescence microscopic analysis. The caspases are the most important apoptotic executors which are responsible for the activation and deactivation of other cellular proteins involved in the apoptotic process or directly involved in cell dismantling. In the cytosol, the activation of caspases can be regulated by another protein called inhibitor of apoptosis protein (IAP). In order to investigate the influence of the XIAP on the apoptosis induced by serum deprivation, we have transfected the hybridoma C_2E_7 cells with XIAP gene. In a culture without any serum supplementation, the XIAP cell was able to maintain greater than 75% viability for over 4 days, while the viability of control cells decreased to below 6%. No further increment was observed in the total cell number for the control cell under serum free conditions, while the XIAP cell recorded a 13% increase in total cell number. This result shows that the XIAP cell is still able to proliferate without the presence of any serum components.
机译：在血清剥夺病症中培养的杂交瘤C_2E_7细胞仅在分批培养中不超过5天，而含有10％的血清的培养表现出远优异的增殖和生存性。血清补充的培养物能够在分批培养中存活超过9天，并记录最大细胞密度的6.6倍。在分批培养5天后，对照培养的可行性仍然超过50％，而血清剥夺细胞的活力降至17％。细胞死亡的机制主要通过细胞凋亡，其在DNA凝胶和繁殖微观分析上以经典梯型模式示出。胱天蛋白酶是最重要的凋亡考官，其负责凋亡过程中其他细胞蛋白的激活和失活，或者直接参与细胞拆卸。在细胞溶溶胶中，胱天蛋白酶的活化可以被称为凋亡蛋白（IAP）抑制剂的另一种蛋白质调节。为了探讨XIAP对血清剥夺诱导的凋亡的影响，我们用XIAP基因转染了杂交瘤C_2E_7细胞。在没有任何血清补充的文化中，XIAP细胞能够在4天内保持大于75％的活力，而对照细胞的活力降低至低于6％。在无血清条件下，在对照细胞的总细胞数中没有观察到进一步的增量，而XIAP细胞记录总细胞数增加13％。该结果表明，在没有任何血清组分的情况下，XIAP细胞仍然能够增殖。

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