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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Biosynthesis of amino acids sulfur in Saccharomyces cerevisiae is affected by fermentation conditions in beer production
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Biosynthesis of amino acids sulfur in Saccharomyces cerevisiae is affected by fermentation conditions in beer production

机译：酿酒酵母酿酒酵母中氨基酸硫的生物合成受到啤酒生产中发酵条件的影响

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Beer elaboration process is still considered, at least in part, an art. It is well known that both yeast and wort play an important role in the quality of final product, because several wort-compounds have influence in aroma and taste. In response to wort composition, the metabolic pathways can be modified in the yeast, and final product could result with high concentration of undesirable volatile sulfur compounds (VSC), mainly H_2S and SO_2. In this work we analyzed the expression of genes involved in the biosynthesis of VSC in Saccharomyces cerevisiae growing under different fermentation conditions. We designed two kinds of wort: one produced with high content of malt (above 50%) and other with low content of malt (below 50%), and were inoculated with yeast previously maintained at 4°C/4h and 18°C/18h, respectively. Yeast was collected at second day of fermentation and gene expression analysis was done using DNA microarrays. Biosynthesis pathway of sulfur amino acids was affected since the genes MUP3, HOM2, MET2, SER2 and MET3 were up regulated, while genes such as MET13, MET6 and MHT1 were down regulated. Moreover, H_2S production was increased in low malt worts. Our results demonstrate that the wort composition plays an important role in the biosynthesis of sulfur amino acids and it could impact the generation of VSC.
机译：至少部分地考虑啤酒培养过程，至少部分地是一部分。众所周知，酵母和麦芽汁在最终产品的质量上发挥着重要作用，因为几种麦芽类化合物对香气和味道有影响。响应于麦芽汁组合物，可以在酵母中修饰代谢途径，最终产物可能导致高浓度的不希望的挥发性硫化合物（VSC），主要是H_2S和SO_2。在这项工作中，我们分析了在不同发酵条件下生长的酿酒酵母生长的VSC生物合成中所涉及的基因的表达。我们设计了两种麦芽汁：用高麦芽含量（高于50％）和其他麦芽含量（低于50％）的麦芽（50％）产生的麦芽汁，并用以前保持在4℃/ 4H和18℃/ 18℃/的酵母接种。 18h分别。在发酵第二天收集酵母，并使用DNA微阵列进行基因表达分析。硫氨基酸的生物合成途径受到抑制基因MUP3，HOM2，MET2，SER2和MET3的影响，而MET13，MET6和MHT1如MED13，MET6和MHT1的基因。此外，在低麦芽麦芽汁中增加了H_2S产量。我们的结果表明，麦芽汁组合物在硫氨基酸的生物合成中起重要作用，并且它可能影响VSC的产生。

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