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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Detection of group I and group II introns in a Mexican Bacillus thuringiensis collection
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Detection of group I and group II introns in a Mexican Bacillus thuringiensis collection

机译：墨西哥芽孢杆菌收集中I和II组内含子的检测

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Group I and II introns are non-coding sequences able to excise themselves from precursor mRNA by self-splicing. Both groups are ribozymes and may also code Open Reading Frames (ORF) for enzymes involved in the homing process; the Homing Endonuclease (HEase) in group I introns and maturase for group II. In this study, Mexican Bacillus thuringiensis strains belong to the Universidad Autónoma de Nuevo León collection were evaluated for the presence of these introns. For this propose, specific primers were designed for group I introns inserted in the ribonucleotide reductase operon and group II introns B.th.I, Bth.I2and B.th.I3. The PCR results obtained with primers for group I, was a DNA band of 600 bp for most strains (fifty four) and one of 200 bp for six one. The nucleotide sequence of 600 pb DNA band revealed more than 95% homology with the amplicons and group I introns reported before. Concern to group II introns, we can not see PCR products correspond to expect DNA band size.
机译：I和II组内含子是非编码序列，其能够通过自拼接从前体mRNA中切除。两组都是核酶，也可以编码归巢过程中涉及的酶的开放阅读框架（ORF）; II族内含子和天然酶中的归巢内切核酸酶（释放）。在本研究中，墨西哥芽孢杆菌菌株属于UsidaIdaMaoma De NuevoLeón系列，评估了这些内含子的存在。对于该提出，设计了针对插入核糖核苷酸还原酶卷曲和II族内含子B.Th.i，Bth.i2和B.Th.i3的I II族内含子的基团Introns的特异性引物。用基团的引物获得的PCR结果是大多数菌株（五十四）的600bp的DNA带，六个六个菌株中的一种。 600pb DNA带的核苷酸序列揭示了95％的同源性与Introns以前报道的分组和组。对II组内含子的关注，我们看不到PCR产品对应于预期DNA带尺寸。

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