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首页> 外文会议>Conference on advanced microscopy techniques IV >Nanosecond Two-photon excitation fluorescence imaging with a multi color fiber MOPA laser
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Nanosecond Two-photon excitation fluorescence imaging with a multi color fiber MOPA laser

机译：纳秒二光子激发荧光成像与多色纤维MOPA激光器

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A system is presented that uses a fiber based Master Oscillator Power Amplifier (MOPA) with nanosecond-range pulses for two-photon excitation fluorescence (TPEF) imaging. The robust laser in the extended near infrared is based on an actively modulated electro-optical modulator (EOM), enabling free synchronization of the pulses to any other light source or detection unit. Pulses with a freely programmable duration between 0.4 and 10 ns are generated and then amplified to up to kilowatts of peak power with ytterbium doped fiber amplifiers (YDFA). Since we achieve peak power and duty cycles comparable to standard femto- and picosecond setups, the TPEF signal levels are similar, but realized with a robust and inexpensive fiber-based setup. The delivery fiber is further used as an optional, electronically controllable Raman shifter to effectively shift the 1064 nm light to 1122 nm and to 1186 nm. This allows imaging of a manifold of fluorophores, like e.g. TexasRed, mCherry, mRaspberry and many more. We show TPEF imaging of the autofluorescence of plant leaves of moss and algae, acquired in epi-direction. This modular laser unit can be integrated into existing systems as either a fiber-based, alignment free excitation laser or an extension for multi-modal imaging.
机译：提出了一种系统，该系统使用基于主振荡功率放大器（MOPA）与纳秒范围脉冲对双光子激发荧光（TPEF）成像的纤维。在坚固的激光扩展近红外线是基于主动调制的电光调制器（EOM），使脉冲的自由同步的任何其他光源或检测单元。用0.4和10纳秒之间的可自由编程持续时间的脉冲被产生，然后放大到高达镱掺杂光纤放大器（YDFA）的峰值功率千瓦。由于我们达到峰值功率和占空比与标准飞秒和皮秒设置中，TPEF信号电平是类似的，但具有强大的和廉价的基于光纤的设置来实现。传输光纤，还用于作为任​​选，电子可控拉曼位移器以有效的1064nm的光转变为1122纳米和1186至纳米。这允许荧光团的歧管的成像，例如像TexasRed，mCherry的，mRaspberry等等。我们发现苔藓植物的叶子和藻类，在外延方向取得的自发荧光的TPEF成像。这种模块化激光单元可以被集成到现有系统中，可以是基于光纤的，对准自由激发激光或用于多模态成像的延伸。

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