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首页> 外文会议>Annual meeting exposition of the Controlled Release Society >TATp-liposomes as a tool for in vitro transfection of antigen-presenting cells and in vivo transfection upon vaccination against Bovine Herpes Virus 1 (BoHV-1)
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TATp-liposomes as a tool for in vitro transfection of antigen-presenting cells and in vivo transfection upon vaccination against Bovine Herpes Virus 1 (BoHV-1)

机译：TATP-脂质体作为用于体外转染抗原呈递细胞的工具，并在对牛疱疹病毒1（BOHV-1）的疫苗接种时进行体内转染

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One of the most promising ways to modulate the immune response is with gene therapy of the immune system (IS), including the use of DNA vaccines, which can generate an immune response after cell transfection. However, antigen-presenting cells (APC), the key-linkers between the innate and the adaptive immunity, are very resistant to transfection. To enhance the transfection efficiency of mouse APC, derived from the bone marrow or spleen, we used TATp-modified liposomal carriers (TATp-L) for the intracellular delivery of plasmids encoding for EGFP (pEGFP) or Bovine Herpes Virus I glycoprotein D (pCI-gD). Liposomes were also tested as DNA vaccine carriers. TATp-lipoplexes allowed for significantly enhancement of both the uptake and transfection of APC in vitro, and they were shown to be efficient in eliciting T lymphocyte mediated cytotoxicity in vivo after vaccination.
机译：调节免疫应答的最有希望的方法之一是免疫系统的基因治疗（是），包括使用DNA疫苗，其在细胞转染后可以产生免疫应答。然而，抗原呈递细胞（APC），先天内和自适应免疫之间的关键接头是非常抗性的。为了增强源自骨髓或脾脏的小鼠APC的转染效率，我们使用TATP改性的脂质体载体（TATP-L）用于编码EGFP（PEGFP）或牛疱疹病毒I糖蛋白D（PCI）的细胞质粒的细胞内递送（PCI -GD）。脂质体也被视为DNA疫苗载体。允许在体外显着提高APC的摄取和转染的TATP-Lipoplexes，并且它们被证明在疫苗接种后在体内引发T淋巴细胞介导的细胞毒性有效。

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