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Dynamic OCT Measurements of Corneal Biomechanical Properties after UV Cross-unking in the Rabbit

机译:动态OCT测量兔紫外线交叉刺激后角膜生物力学性能。

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Structural properties of the cornea determine the shape and optical quality of the eye. Keratoconus, a structural degeneration of the cornea, is often treated with UV-induced collagen cross-linking to increase tissue resistance to further deformation and degeneration. Optimal treatments would be customized to the individual and consider preexisting structural properties as well as the effects induced by treatment and this requires the capability to noninvasively measure tissue properties. The purpose of this study is to use novel methods of optical elastography to study the effects of UV-induced corneal collagen cross-linking in the rabbit eye. Low-amplitude (<1μm) elastic flexural waves were generated using focused air-pulse stimulation. Elastic wave propagation was measured over a 10×10mm area using Phase Stabilized Swept Source Optical Coherence Elastography (PhS-SSOCE) with a sensitivity of ~ 10 nm. Wave amplitude and velocity were computed and compared in tissues before and after UV cross-linking. Wave amplitude was decreased by the cross-linking treatment, while wave velocity was greater in cross-linked tissue than it was in the untreated cornea. Decreased wave amplitude and increased wave velocity after cross-linking is consistent with increased tissue stiffness. This was confirmed by conventional mechanical tension testing. These results demonstrate that the combination of the PhS-SSOCE and focused air pulse stimulation is capable of measuring low amplitude tissue motion and quantifying corneal stiffness.
机译:角膜的结构特性决定了眼睛的形状和光学质量。圆锥角膜是角膜的结构性变性,通常用紫外线诱导的胶原蛋白交联来治疗,以增加组织抵抗进一步变形和变性的能力。最佳治疗将针对个人进行定制,并考虑预先存在的结构特性以及治疗引起的影响,这需要无创地测量组织特性的能力。这项研究的目的是使用光学弹性成像的新方法来研究紫外线诱发的兔眼角膜胶原交联的作用。使用聚焦空气脉冲刺激产生低振幅(<1μm)的弹性弯曲波。使用相位稳定的扫频源光学相干弹性成像技术(PhS-SSOCE),在10×10mm的区域内测量了弹性波的传播,灵敏度为〜10 nm。计算并比较了紫外线交联前后组织中的波幅和速度。交联处理降低了波幅,而交联组织中的波速大于未处理的角膜。交联后波幅减小和波速增加与组织刚度增加是一致的。这通过常规的机械张力测试得到了证实。这些结果表明,PhS-SSOCE和聚焦空气脉冲刺激的组合能够测量低振幅组织运动并定量角膜硬度。

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