首页> 外文会议>Photons Plus Ultrasound: Imaging and Sensing 2006; Progress in Biomedical Optics and Imaging; vol.7, no.9 >Technical Considerations in Quantitative Blood Oxygenation Measurement Using Photoacoustic Microscopy in Vivo
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Technical Considerations in Quantitative Blood Oxygenation Measurement Using Photoacoustic Microscopy in Vivo

机译:体内使用光声显微镜进行定量血氧测定的技术注意事项

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Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO_2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μ_a Λ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO_2 values for in vivo measurements.
机译:使用在570-600 nm范围内的峰幅度光谱PA测量,我们可以量化血液中的氧合水平。可见光照明可将PA测量值的反演误差降至最低。由于在这种光学方式下的高血液吸收,因此还提高了信噪比,并减少了来自光学散射的影响。为了获得正确且独立于容器尺寸的SO_2测量结果,超声换能器的中心频率必须足够高,以满足关系μ_aΛ<1,即对于选定的光波长区域,其高于25 MHz,尽管较低频率的换能器可能校正光吸收光谱后产生正确的结果。但是,需要更多的努力来获得用于体内测量的准确的SO_2值。

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