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首页> 外文会议>Plasma for bio-decontamination, medicine and food security >Damages of Biological Components in Bacteria and Bacteriophages Exposed to Atmospheric Non-thermal Plasma
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Damages of Biological Components in Bacteria and Bacteriophages Exposed to Atmospheric Non-thermal Plasma

机译：大气非热等离子体对细菌和噬菌体生物成分的损害

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Mechanism of inactivation of bio-particles exposed to dielectric barrier discharge, DBD, has been studied using E. coli and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E.coli cells proved that Non-thermal Plasma, NTP causes a prominent protein damages without cutting peptide bonds. We have developed a biological assay which evaluates in vitro DNA damage of the bacteriophages. Bacteriophage X having double stranded DNA was exposed to DBD, then DNA was purified and subjected to in vitro DNA packaging reactions. The re-packaged phages consist of the DNA from discharged phages and brand-new coat proteins. Survival curves of the re-packaged phages showed extremely large D value (D = 25 s) compared to the previous D value (D=3 s) from the discharged phages. The results indicate that DNA damage hardly contributed to the inactivation, and the damage in coat proteins is responsible for inactivation of the phages. M13 phages having single stranded DNA were also examined with the same manner. In this case, damage to DNA was as severe as that of the coat proteins.
机译：已经使用大肠杆菌和噬菌体研究了暴露于介电势垒放电DBD的生物粒子失活的机理。在灭活过程中监测不同生物成分的状态。对导入大肠杆菌细胞的绿色荧光蛋白GFP的分析证明，非热血浆NTP会引起显着的蛋白损伤，而不会切断肽键。我们已经开发了一种生物测定法，可评估噬菌体的体外DNA损伤。将具有双链DNA的噬菌体X暴露于DBD，然后纯化DNA并进行体外DNA包装反应。重新包装的噬菌体由排出的噬菌体的DNA和全新的外壳蛋白组成。重新包装的噬菌体的存活曲线显示，与排出噬菌体的先前D值（D = 3 s）相比，D值非常大（D = 25 s）。结果表明，DNA损伤几乎不会导致失活，而外壳蛋白的损伤是噬菌体失活的原因。还以相同的方式检查了具有单链DNA的M13噬菌体。在这种情况下，对DNA的损害与外壳蛋白一样严重。

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