目的 观察ω-3 多不饱和脂肪酸对严重烧伤大鼠肠道炎症反应的影响,初步探讨其对肠道功能调节的机制.方法 清洁级SD大鼠72只,随机分为3组:正常组(A组)、力能对照组(B组)和鱼油治疗组(C组).复制大鼠30%TBSA Ⅲ度烧伤模型,A组大鼠经尾静脉注射生理盐水2 ml/(kg·d),B组每天定时经尾静脉注射20%力能中长链脂肪乳 2 ml/(kg·d),C组在相同时间点经尾静脉注射与B组等氮等热量的尤文ω-3鱼油脂肪乳2 ml/(kg·d).分别观察伤前(A组)、伤后(B、C组)1、4、7、10 d血清二胺氧化酶(DAO)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平;免疫组织化学染色法观察肠组织TNF-α的表达.结果 B、C两组在伤后各时相点血清DAO、TNF-α、IL-6水平显著高于伤前(P<0.01);C组血清DAO、TNF-α、IL-6水平较B组降低,在伤后4、7、10 d与B组比较差异有显著性(P<0.05 或P<0.01).与B组相比,C组TNF-α活性明显减弱.结论 ω-3鱼油脂肪乳降低烧伤后血清DAO水平,改善烧伤后肠黏膜损伤,保护肠黏膜屏障功能.ω-3鱼油脂肪乳减轻烧伤后促炎症介质TNF-α、IL-6的释放,抑制烧伤后全身炎症反应的程度.%To investigate the effects of ω3 polyunsaturated fatty acids( go-3 PUFAs ) on intestinal inflammatory responses following severely burned rats,and preliminary explore the adjustment mechanism of ω-3 PUFAs on the intestinal function. Methods A total of 72 SD rats with 30% total body surface area ( TBSA ) fully thickness burned were randomly divided into 3 groups: the normal control group ( A group ), the experimental group ( B group ) and the treatment group ( C group ). Rats of group A were injected NS with 2 ml/( kg · D ) by tail vein, Rats of group B were injected 20% middle-long-chain fatty emulsion with 2 ml/( kg · D ) by tail vein, Rats of group C were injected go-3 fish oil with 2 ml/( kg · D ) by tail vein at the same time for each day. The levels of serum di-amine oxidase ( DAO ), tumor necrosis factor-alpha ( TNF-α ), inter leukins-6( IL-6 ) were measured before injury group ( Group A ) and after injury groups ( Group B, C ) in 1 ,4,7,10 d respectively. Expression of TNF-a in intestinal tissue was detected by immunohistochemical staining. Results Serum levels of DAO,TNF-a, IL-6 were significantly higher than those pre-injury at each time point after injury in group B and C( P <0. 01 ). The levels of DAO, TNF-α, IL-6 in group C were lower than those in group B , and were significantly different in 4,7 ,10 d after injury( P <0. 05 or P <0. 01 ). Compared with the group B,TNF-α activity was significantly decreased in group C. Conclusion go-3 PUFAs can reduce serum DAO level, improve intestinal mucosa injury, and protect the function of intestinal mucosal barrier after burn, ω-3 PUFAs can reduce the releasing of pro-inflammatory media, such as TNF-a and IL-6, inhibit the extent of the systemic inflammation.
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