Objective To explore biomarker of Gemcitabine (GEM) chemosensitivity in non-small cell lung cancer (NSCLC). Methods GEM IC50 of 30 NSCLC tissues were detected by susceptibility testing in vitro, mRNA expression of hENT1, dCK ,RRM1 and RRM2 were determined by Realtime-PCR. Results hENT1, dCK, RRM1 or RRM2 expression levels in individual gene had no relationship with the GEM IC50 value (P > 0.05), however, ratio of (hENT1ídCK)/(RRM1íRRM2) was negatively correlated with GEM IC50 value (P < 0.001). Conclusion Ratio of (hENT1ídCK)/(RRM1íRRM2) may predict GEM chemosensitivity in NSCLC.%目的：探讨非小细胞肺癌(NSCLC)吉西他滨(GEM)化疗敏感性预测靶标。方法30例NSCLC组织，体外药敏试验明确GEM IC50值；Realtime-PCR检测GEM代谢酶hENT1、dCK、RRM1和RRM2 mRNA表达水平。结果 hENT1、dCK、RRM1和RRM2单个基因表达量与GEM IC50值无关(P>0.05)；(hENT1×dCK)/(RRM1×RRM2)值与GEM IC50值呈负相关(P<0.001)。结论(hENT1×dCK)/(RRM1×RRM2)值可作为GEM化疗敏感性预测的潜在标志物。
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