AIM:To examine the effects of toll like receptor 4 (TLR4)on the proliferation of human perio-dontal ligament cells (hPDLCs).METHODS:Primary hPDLCs were cultured by tissue block enzymolytic method. Cells of Passege 3 were transfected with NC-shRNA and TLR4-shRNA respectively,cells without treatment served as the controls.72 hours after treatment,TLR4 mRNA expression was examined by real-time quantitative polymerase chain reaction (RT-PCR).MTT assay was used to test the proliferation of hPDLCs.RESULTS:TLR4 mRNA expres-sion in the TLR4-shRNA group was significantly decreased than that in other groups (P<0.05).MTT assay showed that the OD values of TLR4-shRNA group at 48 and 72 h were significantly lower than those of the NC-shRNA group or blank control group(P<0.05).CONCLUSION:TLR4 interference suppresses cell proliferation of hPDLCs.%目的：了解Toll样受体4对人牙周膜细胞增殖的影响。方法：以改良组织块法体外获得人牙周膜细胞，取生长良好的第3代细胞随机分为3组：空白对照组、NC对照组（NC－shRNA转染组）和TLR4－shRNA转染组，并于转染48 h 后用倒置荧光显微镜观察转染效果、用荧光实时定量 PCR检测各组细胞中TLR4mRNA的表达水平；最后将3组细胞分别接种于96孔细胞培养板进行培养，分别于培养24、48、72 h用MTT法检测各组细胞的增殖能力。结果：TLR4－shRNA可明显降低人牙周膜细胞的TLR4mRNA表达水平（P＜0．05）；MTT检测结果显示，TLR4－shRNA转染组在常规培养48、72 h的OD值均明显低于空白对照组和NC对照组（P＜0．05），而NC对照组的OD值在72 h时明显低于空白对照组（P＜0．05）。结论：TLR4对牙周膜细胞的增殖有一定调控作用，转染慢病毒后该作用增强。
展开▼
