Objective To explore the protection effect and the mechanism of sodium ferulate on renal interstitial fi-brosis in rats after unilateral ureteral obstruction( UUO ). Methods Forty-five Sprague Dawley ( SD ) rats were divided randomly and evenly into sham group, UUO model group, and sodium ferulate treatment group. The subjets were sacrificed respectively on 3 , 7, and 14 day postoperatively, and their operated kidneys were harvested for comparison on pathology. Then administered immunohistochemical staining for the expression of TGF-βl, α-SMA, and p-p38MAPK using SP, and made statistical analysis by means of SAS software finally. Results Compared with sham operated group, the expression of TGF-βl, p-p38MAPK,and α-SMA increased remarkably in UUO model group and treatment group( P <0.05 ); while compared with the UUO model group, the expession of TGF-β1, p-p38MAPK, and α-SMA decreased notably in treatment group( P < 0.05 ). And there was a tight positive correction between the expession of TGF-β1, p-p38MAPK,and α-SMA ( TGF-β1 and p-p38MAPK: r =0.745, P <0.05; p-p38MAPK and α SMA: r =0.659, P <0.05; TGF (31 and a-SMA: r =0.770, P < 0.05 ). Conclusion The sodium ferulate ccould decrease the expression of a-SMA by way of TGF-β1/p-p38MAPK, so as to alleviate renal interstitial fibrosis.%目的 探讨阿魏酸钠对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的保护作用及机制.方法 将45只SD大鼠随机分为假手术组、模型组、阿魏酸钠治疗组.分别于UUO术后3、7、14 d处死动物并取梗阻肾组织,比较病理改变;用SP法检测TGF-β1、p-p38MAPK、α-SMA的表达,并比较分析.结果 与假手术组相比,模型组TGF-β1、p-p38MAPK、α-SMA的表达显著升高(P<0.05)且随时间延长,升高更显著;与模型组相比,治疗组TGF-β1、p-p38MAPK、α-SMA的表达显著降低(P<0.05);且TGF-β1、p-p38MAPK、α-SMA的表达呈现正相关性:TGF-β1与p-p38MAPK(r=0.745,P<0.05);p-p38MAPK与α-SMA(r=0.659,P<0.05);TGF-β1与α-SMA(r=0.770,P<0.05).结论 阿魏酸钠可能通过TGF-β1/p-p38 MAPK通路,下调α-SMA的表达,从而减轻大鼠肾间质纤维化.
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