Objective: To investigate the effectiveness of recombinant retrovirus vector in gene therapy. Methods: The retroviral vector PLXSN-S was constructed and transferred into PA317 by means of electroporation, then HepG2, P815, and EL4 cells were infected with the pseuovirus produced from PA317, which highly expressed HBsAg. HBsAg expression was tested by RT-PCR and ELISA. Results: HBsAg was expressed variously in the eukaryotic cells mentioned above. HBsAg (A value) of the cell supernatants (48h) were 0.92, 0.09, 0.47, respectively. Conclusion: The vector used in this study is an effective one to carry genes of interest to target cells and it may be useful in the test for gene therapy.%目的：探索逆转录病毒载体在基因治疗中的应用。 方法：用DNA重组技术构建HBV-S基因重组逆转录病毒载体，电穿孔转染PA317后，筛选高表达克隆，用假病毒颗粒感染HepG2、P815和EL4细胞，分别用RT-PCR及ELISA法检测目的基因表达。 结果：HBsAg在上述细胞中获得不同程度的表达，细胞上清液（48h）中HBsAg含量（吸光度值）分别为0.92、0.09和0.47。结论：逆转录病毒用载体，其目的基因在细胞内稳定表达，是一种高效的基因转移系统，适用于基因治疗实验。
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