目的：探究JAK－STAT信号通路及炎症因子IL－1β、IL－6是否参与X射线诱导的PC12细胞辐射损伤。方法：采用X射线分别以2、4和8 Gy剂量照射PC12细胞，照射后24 h通过酶联免疫法检测IL－1β和IL－6的表达水平；Western blot检测p－JAK1、p－JAK2、p－STAT1、p－STAT3和p－STAT5的蛋白水平。结果：与正常对照组相比，细胞经不同剂量X射线照射24 h后，IL－1β和IL－6的表达水平均升高，且与辐照剂量呈剂量依赖性；p－JAK1、p－JAK2、p－STAT1、p－STAT3和p－STAT5的蛋白水平均升高，且上调程度与辐照剂量呈剂量依赖性。结论： JAK－STAT信号通路、IL－1β和IL－6可能参与X射线照射诱导PC12细胞的损伤调控。%AIM:To investigate the role of JAK-STAT pathway , IL-1βand IL-6 in the PC12 cells with X-ray irradiation.METHODS:The PC12 cells were irradiated with X-ray at doses of 2, 4 and 8 Gy.After 24 h, the levels of IL-1βand IL-6 were detected by ELISA .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 were measured by Western blot .RESULTS:Compared with control group , the levels of IL-1βand IL-6 increased .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 increased with the doses of X-ray exposed.CONCLUSION:JAK-STAT signaling pathway , IL-1βand IL-6 play a role in the injury of PC12 cells with X-ray irradiation.
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