AIM: To establish a method to produce animal model of diabetic nephropathy (DN) in New Zealand rabbits using alloxan (ALX). METHODS: Twenty male New Zealand rabbits were randomly divided into 2 groups: 8 rabbits in control group were fed with conventional feed such as buffer solution ; 12 in diabetes milleuts (DM) group were fed with high - sugar and high - fat diet (conventional feed plus 5% sucrose, 5 % pork fat and 1 % cholesterol) for 2 weeks , then intravenous injection of ALX at 50 mg/kg was given , and another dosage of 100 mg/kg ALX was intravenously injected after 48 h. The levels of blood glucose , blood cholesterol, triglyceride , urine microalbumin , and serum creatinine were detected before and 12 weeks after the processes of modeling. After 12 weeks , the rabbits were sacrificed , and the kidneys were taken for pathological examination . RESULTS : The successful model of DN in the rabbits had the characteristics of high blood glucose , abundant urine microalbumin (P < 0. 01) , and corresponding morphological and functional changes of the kidneys. CONCLUSION: Rabbits with high - sugar and high - fat diet plus twice intravenous injection of ALX at a total dose of 150 mg/kg (50 mg/kg and 100 mg/kg, respectively) shows low mortality and stable diabetes mellitus state . This method induces a typical DN model in 12 weeks.%目的:探讨小剂量四氧嘧啶(ALX)建立新西兰兔糖尿病肾病(diabetic nephropathy,DN)模型的可行性.方法:雄性新西兰兔20只,8只为对照组,给予常规饲料,12只为糖尿病组,给予高糖高脂饮食(常规饲料加5%蔗糖、5%猪油和1%胆固醇)喂养2周,再加用小剂量ALX耳缘静脉注射,分别检测造模前与造模12周后兔血糖、血脂、尿蛋白和肾功能指标,并于12周后进行组织形态学观察.结果:建模成功动物具有"多饮、多食、多尿、体重减轻"的特点,并出现DN相应的形态及功能改变.结论:高脂高糖饮食加ALX药物诱导的方法,12 周时可成功制备新西兰兔2型DN模型.
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