目的：探讨灯盏花素对胰岛素抵抗（IR）大鼠肝脏的保护作用。方法采用高脂高糖饲料饲养12周建立IR大鼠模型，成模后随机均分为灯盏花素高剂量组（HD组）、灯盏花素低剂量组（LD组）、模型组（MO组），各组以相应的药物干预2周。另设正常组（NO组），普通饲料喂养，不给药物干预。通过测定大鼠血清空腹胰岛素（FINS）和空腹血糖值（FBG）来计算胰岛素敏感指数（ISI）；检测肝脏组织中丙二醛（MDA）、超氧化物岐化酶（SOD）的活性以观察药物对大鼠肝脏氧化和抗氧化的作用；运用苏木精-伊红（HE）染色制片检测大鼠肝脏组织病理学改变；油红O染色法观察肝脏组织脂肪沉积和脂肪浸润。结果与NO组相比，MO组大鼠ISI明显降低（P＜0．01）；肝脏中MDA含量明显升高，SOD活性明显下降（P＜0．01）；HE形态显示，与NO组相比，MO组大鼠肝细胞明显脂肪变性，经过灯盏花素治疗后，与MO组相比，HD组及LD组大鼠肝细胞脂肪变性明显好转；油红O染色结果显示，MO组大鼠肝脏组织脂肪沉积明显，强度大（P＜0．01）；与MO组相比，HD组和LD 组大鼠肝脏脂肪沉积明显好转，HD组大鼠SOD活性较MO组明显升高（P＜0．01），MDA的含量较MO组明显下降（P＜0．01）。结论灯盏花素可改善IR，对IR大鼠肝脏具有保护作用，其机制可能与改善IR和抗氧化有关。%Objective To determine the hepatoprotective effect of breviscapine in rats with insulin resistance (IR). Methods IR was modeled in rats via a 12-week feeding on high-sugar high-fat diet. Then the rats were ran-domly divided into high-dose Breviscapine group (HD group), low-dose Breviscapine group (LD group) and model group (MO group). The HD and LD groups were intervened with Breviscapine for two weeks. A normal group (NO group) on normal diet was set and did not receive any intervention. Serum levels of fasting insulin (FINS) and fasting blood glucose (FBG) were measured to calculate the insulin sensitivity index (ISI). Activity of liver malondialdehyde (MDA) and superoxide dismutase (SOD) was determined to observe the effects of Breviscapine on rat liver oxidation and anti-oxidation. The pathological changes in rat liver tissue were studied using HE staining. Oil Red O staining was used to observe the fat deposition and fatty infiltration in liver tissue. Results Compared with the NO group, MO rats had significantly lower ISI (P<0.01), significantly increased liver MDA and decreased SOD activity (P<0.01). HE-stained histology showed that, compared with the NO group, MO rats had apparent hepatic steatosis. After treatment with Breviscapine, HD and LD groups showed remarkable improvement in liver steatosis compared with the MO group. Oil red staining showed apparent and heavy fat deposition in MO rat livers (P<0.01). Compared with the MO group, HD and LD groups showed significant improvement in liver fat deposition; significantly higher SOD activity (P <0.01) and lowered MDA content (P<0.01) was found in rat liver of HD group than as found for the MO group. Conclusion Breviscapine may be hepatoprotective in IR rats, probably via a mechanism related to improvement of insulin resis-tance and anti-oxidation.
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