研究全缘栝楼多糖和提取物不同极性部位体外抗氧化活性。采用清除羟自由基（OH·）、[2，2＇-连氨-（3-乙基苯并噻唑林-6-磺酸）二氨盐]自由基（ABTS+·）、DPPH测定法以及Fe3+还原/抗氧化能力（FRAP）法，评价全缘栝楼多糖和提取物不同极性部位的抗氧化能力。全缘栝楼多糖和提取物不同极性部位清除DPPH·能力均强于抗坏血酸（IC50=1.22 mg/mL），且多糖（IC50=0.030 mg/mL）和乙酸乙酯层（IC50=0.082 mg/mL）比茶多酚强（IC50=0.118 mg/mL）；多糖（IC50=0.031 mg/mL）清除OH·能力比茶多酚（IC50=0.032 mg/mL）和抗坏血酸（IC50=0.044 mg/mL）强，而石油醚层（IC50=0.033 mg/mL）和乙酸乙酯层（IC50=0.038 mg/mL）强于抗坏血酸；清除ABTS+·能力均强于茶多酚（IC50=0.416 mg/mL），且多糖（IC50=0.008 mg/mL）强于抗坏血酸（IC50=0.011 mg/mL）；Fe3+还原/抗氧化能力均强于茶多酚（FRAP =1310.8μmol/g），但比抗坏血酸弱（FRAP=31469μmol/g）。全缘栝楼多糖和提取物不同极性部位有较强的抗氧化能力，为其生物活性的深入研究提供了参考依据。%To study the antioxidant activity of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera. The antioxidant activities of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera were evaluated by DPPH free radical scavening assay , and hydroxyl free radical (OH·) scavenging assay, and [2, 2'-Azino-bis (3-ethylbenzo thia zoline-6-sulfonic acid)]diammonium salt radical (ABTS+·)and ferric reducing antioxidant power (FRAP)assay. DPPH free radical scavening activity of polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera were higher than VC (IC50 1.22 mg/mL) , and polysaccharides (IC50 0.030 mg/mL) and ethylacetate fraction(IC50 0.082 mg/mL) were higher than tea polyphenol(IC50 0.118 mg/mL). OH·scavenging activity of polysaccharides(IC50 0.031 mg/mL) were higher than tea polyphenol (IC50 0.032 mg/mL) and VC(IC50 0.044 mg/mL),and petroleum ether(IC50 0.033 mg/mL) and ethylacetate fraction (IC50 0.038 mg/mL)were higher than VC. ABTS+· scavenging activity and ferric reducing antioxidant capacity of polysaccharides and different polarity chemical fractions of extract were higher than tea polyphenol were higher than tea polyphenol (IC50 0.416 mg/mL, FRAP 1 310.8μmol/g ),and ABTS+·scavenging activity of polysaccharides (IC50 0.008 mg/mL) higher than VC (IC50 0.011 mg/mL). Polysaccharides and different polarity chemical fractions of extract from Trichosanthes ovigera had strong antioxidative activity ,this conclusion would provide important evidences for the further study of the bioactivity.
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