通过响应面分析对桦褐孔菌抗氧化活性成分的提取工艺进行了优化，并对提取物的铁离子还原能力（FRAP）、DPPH自由基清除能力进行了评价。结果表明，最佳提取工艺为料液比1∶20（g/mL），乙醇浓度65%，提取温度85℃，提取时间2.5 h。根据最佳提取工艺，得到桦褐孔菌抗氧化活性成分提取物得率为21.6%，其多酚含量为12.04 mg/g，铁离子还原能力测试（FRAP）值为1.42 mmol/g样品，提取物浓度为400μg/mL时DPPH自由基清除率为78.05%。加热实验表明，桦褐孔菌提取物具有一定的热稳定性。%The extracting technology of crude antioxidants from Inonotus obliquus was studied and response surface methodology (RSM) was applied to optimize the process. And antioxidant activities of extracts from Inonotus obliquus were evaluated by FRAP assay and DPPH radical scavenging capacity. The optimal extracting conditions were ratio of materials to solvent 1∶20 (g/mL), concentration of ethanol 65%, extraction temperature 85 ℃ and extraction time 2 . 5 h . Under these conditions , extraction yield of antioxidants was 21 . 6 %, 12.04 mg/g polyphenol could be obtained. The Inonotus obliquus extracts had a strong antioxidant activity, the FRAP value, DPPH radical scavenging capacity with a concentration of 400 μg/mL were 1.42 mmol/g sample (FeSO4·7H2O equivalents) and 78.05 %, respectively. Characterization of the Inonotus obliquus extracts antioxidants proved that those antioxidants were heat-resistant.
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