Objective To isolate and determine the chemical constituents of Cynanchum auriculatum .Method A compound was firstly extracted by methanol, isolated by HPCPC, column chromatography, and HPLC, and then identified by UV, MS, NMR data.Findings One compound was obtained and elucidated as caudatin 3-O-β-D-oleandropyranosyl-(1→4)-β-D-oleandropyranosyl -( 1 → 4 )-β-D-cymaropyranoside. Conclusion The compound was isolated from Cynanchum auriculatumfor the first time.%目的：对萝藦科植物耳叶牛皮消（ Cynacnhum auriculatum Royle ex Wight ）的化学成分进行分离和结构鉴定。方法溶剂提取后采用高效离心分配色谱，大孔吸附树脂柱层析，硅胶柱层析，半制备型HPLC分离纯化，通过理化性质和波谱技术鉴定结构。结果从耳叶牛皮消中分离了1个化合物，鉴定为告达庭3-O-β-D-夹竹桃吡喃糖基-（1→4）-β-D-夹竹桃吡喃糖基-（1→4）-β-D-磁麻吡喃糖苷。结论该化合物为首次从该植物中分离得到。
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