This is the first report of sex reversal induced by injection of androgenic gland (AG) extract in brachyuran crabs.In the present study,the extract of AG from the mud crab Scylla paramamosain and the Chinese mitten crab Eriocheir sinensis was injected into newly developed female (i.e.just after sex differentiation) crabs E.sinensis.Masculinization was observed in the injection with E.sinensis as well as S.paramamosain.AG cross-activity might exist between the two species.Sex reversal could be achieved at a low dosage of 0.14 (E.sinensis) or 0.06 (S.paramamosain) AG equivalent.%该论文首次报道了经由促雄性腺提取物的注射而在蟹类中引起的性逆转现象.此前关于蟹类促雄性腺活性研究的报道极少,而且蟹类的雄性化均是由促雄性腺的移植所产生.本实验中将锯缘青蟹和中华绒螯蟹的促雄性腺提取物分别注射到刚刚完成性别分化的中华绒螯蟹雌性幼蟹体内,此时幼蟹处于4至5期,壳宽为5～8 mm.注射之后,幼蟹经过大约1～2次蜕皮,此时在注射锯缘青蟹以及中华绒螯蟹的促雄性腺提取物的两组实验幼蟹中均能观察到雄性化现象,而注射生理盐水的对照组实验幼蟹中未能观察到相同现象.由此本实验可以证明促雄性腺确实是蟹类的一种雄性激素,注射促雄性腺提取物能引起雌性幼蟹发生性逆转;同时根据锯缘青蟹和中华绒螯蟹的促雄性腺提取物均能引起中华绒螯蟹雌性幼蟹发生性逆转的现象推测,锯缘青蟹和中华绒螯蟹两种间可能存在促雄性腺的交叉活性;不仅如此,性逆转还能在极低的注射剂量下获得,相当于中华绒螯蟹0.14促雄性腺当量和锯缘青蟹0.06促雄性腺当量.
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