目的：探讨乳腺癌 MMP -2、MMP -9表达的临床病理联系及直接阻断 MMP -2、MMP -9对乳腺癌细胞增殖、侵袭及细胞周期的影响。方法：应用免疫组织化学方法检测48例乳腺癌 MMP -2、MMP -9的表达。明胶酶谱法检测乳腺癌 MCF -7细胞 MMP -2、MMP -9的表达。MCF -7细胞接种于预铺 BME 的 Tran-swell 小室上室，与 MMP -2、MMP -9的阻断剂 CTT 共孵育16h，观察阻断 MMP -2、MMP -9对 MCF -7细胞侵袭能力的影响。MCF -7细胞与 CTT 共孵育12h 后，MTT 法检测阻断 MMP -2、MMP -9对细胞增殖能力的影响。MCF -7细胞与 CTT 共孵育24h 后，PI 染色流式法检测阻断 MMP -2、MMP -9对细胞凋亡和细胞周期的影响。结果：48例乳腺癌组织有 MMP -2表达者45例（93.75%），MMP -9表达者38例（79.17%），MMP-2和 MMP -9在发生淋巴结转移组明显高表达（P ﹤0.05）；组织学分级越高，MMP -2和 MMP -9的表达越显著（P ﹤0.05）。明胶酶谱法检测：在 MCF -7培养上清中检测到 MMP -2、MMP -9的表达。在浓度为100μg/ ml 和200μg/ ml 时 CTT 对 MCF -7细胞的侵袭抑制率达到39.5%和61.9%。在终浓度为50、100、200μg/ ml 时，CTT 对 MCF -7细胞的增殖率均无明显下降（P ﹥0.05）。CTT 处理组 MCF -7细胞经流式法检测未见凋亡峰，细胞周期各期未见明显阻滞（P ﹥0.05）。结论：MMP -2、MMP -9与乳腺癌的淋巴结转移密切相关，阻断 MMP -2、MMP -9可有效抑制乳腺癌的浸润和转移。%Objective:To study the clinicoPathological significance of MMP-2/-9 exPressions in breast cancer, and the blocking MMP-2/-9 on breast cancer cells. Methods:The exPressions of MMP-2/-9 were examined in 48 cases of breast cancer by immunohistochemistry. The exPressions of MMP-2/-9 in MCF-7 cells were detected by means of gelatin zymogram. MCF-7 cells were incubated with different concentrations of CTT(MMP-2/-9 blocker)in transwell Precoated with BME for 16h,the invasion of MCF-7 cells was detected. MCF-7 cells were in-cubated with different concentrations of CTT in 96 - microwell Polystyrene Plates for 12h,the Proliferation of MCF-7 cells was detected by means of MTT. Incubated with 200μg/ ml of CTT for 24h,aPoPtosis and cell cycle of MCF-7 cells were detected by means of PI staining flow cytometry. Results:The result of immunohistochemistry:Among 48 breast cancer examPles,there were 45 cases exPressing MMP - 2(93. 75% )and 38 cases exPressing MMP - 9 (79. 17% ). The exPressions of MMP-2/-9 in breast cancer with lymPh node metastasis grouP was higher than no metastasis grouP(P ﹤ 0. 05). The result of gelatin zymogram:MMP-2/-9 could be detected in MCF-7 cell cul-ture suPernatant. Cultured with 100μg/ ml and 200μg/ ml of PePtide CTT,the inhibition ratio of cellular invasion of MCF-7 were 39. 5% and 61. 9% . At all concentrations,the Proliferation ratio of MCF-7 had no significant differ-ence between CTT and blank grouP(P ﹥ 0. 05). The result of flow cytometry showed that no aPoPtotic Peak could be found in MCF-7 cells treated with CTT,and the whole cell cycle had no significant difference between CTT and blank grouP(P ﹥ 0. 05). Conclusion:In breast cancer,the excessive exPressions of MMP-2/-9 may indicate axil-lary lymPh node metastasis,blocking the function of MMP-2/-9 can inhibit the metastasis of breast cancer.
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