Objective : To investigate the promoter methylation status of RUNX3 gene in human breast cancer cell lines,and the relationship between promoter methylation status of RUNX3 gene and RUNX3 expression. Methods: MSP( methylated specific PCR ) was used to measure the promoter methylation status of RUX3 gene in breast cancer cell lines. RT - PCR and Western blot were used to detect the mRNA and protein expressions of RUNX3 gene. Results : Of the 6 cell lines, two ( T47D,MCF7 ) were high - methylated status. RUNX3 mRNA and protein expressions were negative in the two cell lines. RUNX3 mRNA and protein expression were negative in SKBR3 , but the promoter methylation was not detected in this cell line. Conclusion : RUNX3 gene was inactived in breast cancer cell lines because of the promoter methylation. Further research is needed to study other mechanisms.%目的:探讨人乳腺癌细胞系中抑癌基因RUNX3启动子甲基化状态,并分析其与RUNX3基因表达的相关性.方法:运用甲基化特异性PCR(MSP)检测5种乳腺癌细胞系和一种人类正常乳腺细胞系中RUNX3启动子甲基化状态,运用RT-PCR和Western 印迹检测这些细胞系中RUNX3基因mRNA和蛋白的表达.结果:在6种细胞系中,有两种(T47D、MCF7)呈高甲基化状态,并且这两种细胞系的RUNX3 mRNA和蛋白表达阴性.SKBR3中没有检测出RUNX3启动子区的甲基化,但RUNX3 mRNA和蛋白表达阴性.结论:乳腺癌细胞系中RUNX3基因由于启动子区甲基化而失活,但尚有其它失活机制存在,需进一步研究探讨.
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