ABSTRACT:Objective To evaluate the value of COLD-PCR/Sanger in detecting EGFR and KRAS mutations in peripheral blood of patient with non-small cell lung cancer (NSCLC).Meth-ods EGFR and KRAS mutations in peripheral blood samples of 67 patients with NSCLC were detected by COLD-PCR/Sange and common PCR/Sanger,respectively.Results The mutation rates of EGFR and KRAS detected by COLD-PCR/Sanger (43.3% and 16.4%,respectively) were higher than those detected by common PCR/Sanger (26.87% and 5.97%,respectively). These two methods had a better consistency for the detection of EGFR and KRAS mutations (Kappa=0.582 and 0.49,respectively).The mutations mainly occurred in exons 19 and 21.Con-clusion COLD-PCR/Sanger is highly sensitive for detecting EGFR and KRAS mutations in pa-tients with lung cancer.%目的：评价 COLD-PCR／Sanger 法检测晚期非小细胞肺癌（NSCLC）患者外周血 EGFR 和 KRAS 基因突变的价值。方法采用 COLD-PCR／Sanger 对67例晚期 NSCLC 患者外周血中 EGFR 及 KRAS 基因突变进行检测，同时采用普通 PCR／Sanger 法进行平行检测。结果67例样本中，COLD-PCR／Sanger 检测 EGFR 的突变率高于普通 PCR／Sanger 法（43．3％比26．87％，P ＜0．05），主要表现在外显子19、21的突变，二者的一致性较好（Kappa＝0．582）；COLD-PCR／Sanger 检测 KRAS 的突变率高于普通 PCR／Sanger 法（16．4％比5．97％，P ＜0．05），二者的一致性较好（Kappa＝0．49）。结论COLD-PCR／Sanger 是一种高灵敏度检测肺癌患者外周血 EGFR 和 KRAS 基因突变的方法。
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