A new type of nanocomposite bi-layer film modified electrode is successfully constructed based on poly(L-leucine)/DNA using electrodepostion method.The modified electrode is characterized by scanning electron microscope(SEM) and electrochemical impedance spectroscopy(EIS).The electrochemical properties of acetaminophen(AC) on the modified electrode are studied by cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The results show that the modified electrode displays excellent electrochemical catalytic activities toward the oxidation of AC under the optimized conditions.The oxidation current of AC is directly proportional to the concentration of AC in the range of 0.7 to 250 μmol·L-1 and the detection limit is 2.3×10-9 mol·L-1(S/N=3).In addition,the modified electrode shows high sensitivity,stability and reproducibility.Moreover,the modified electrode has been applied for the determination of AC in commercial tablets.%利用电沉积法成功制备了新型的聚L-亮氨酸(Pl-LEU)/DNA双层纳米薄膜修饰电极.采用扫描电镜(SEM)和电化学阻抗(EIS)对修饰电极进行了表征,并用循环伏安(CV)和微分脉冲伏安法(DPV)研究了对乙酰氨基酚(AC)在修饰电极上的电化学行为.结果表明, 在最优条件下, 该修饰电极对AC表现出优越的电催化活性,氧化峰电流与AC浓度在0.7~250 μmol·L-1 范围内呈现良好的线性关系,检测限为2.3×10-9 mol·L-1(S/N=3).此外,该修饰电极还具有较高的灵敏度,较好的稳定性和重现性, 可用于实际药物中AC的快速检测.
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