A bstratc:Objective It is to approach the influence of the combination of folate deficiency and PLK-1 siRNA on the growth of gastric cancer cell lines.Methods After constructing PLK -1 siRNA in vitro, gastric carcinoma cell lines of SGC7901 and BGC823 were transfected with LipofectamineTM 2000.The PLK-1 transfected effect by siRNA was evaluated through detecting messenger RNA and protein of PLK-1 by real-time PCR and Western-blot, respectively.In addition, after transfecting with PLK-1 siRNA, these cell lines of SGC7901 and BGC823 were cultured in media with normal concentrations of folate or folate deficiency for 72 hours.Then, the changes of cell proliferation, apoptosis, cell cycle and the protein expres-sions of Bcl-2 were analyzed.Results Folate deficiency and PLK-1 siRNA inhibited the cell lines proliferation of SGC7901 and BGC823, induced apoptosis and made the cell cycle arrest at G2/M phase, synergistically.They could down regulate the Bcl-2 expression of SGC7901 and BGC823.Conclusions Folate deficiency and PLK-1 siRNA can inhibit the growth of SGC7901 and BGC823 synergistically.The effects may result from inhibiting the expression of BCL-2.%目的：探讨叶酸缺乏和保罗样激酶1（PLK－1）小干扰RNA（ siRNA）的联合作用对胃癌肿瘤细胞的影响。方法 PLK－1 siRNA用LipofectamineTM 2000作载体转染胃癌细胞株 SGC7901和 BGC823， real-time PCR 和 Western blot 验证转染效果；然后将转染的SGC7901和 BGC823细胞株，继续在叶酸缺乏和正常叶酸浓度培养基中培养72 h，检测细胞增殖、凋亡、周期和蛋白Bcl－2的变化情况。结果叶酸缺乏与PLK－1 siRNA具有协同抑制SGC7901、BGC823细胞增殖，促进凋亡和使细胞周期停滞在G2／M期的作用，二者能协同抑制SGC7901、BGC823Bcl －2蛋白表达。结论叶酸缺乏和PLK－1 siRNA均能抑制胃癌细胞SGC7901、BGC823的生长，两者具有协同效应。该效应可能与Bcl－2蛋白的抑制有关。
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