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首页> 美国卫生研究院文献>British Journal of Pharmacology and Chemotherapy >Dual effects of mastoparan on intracellular free Ca2+ concentrations in human astrocytoma cells.
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Dual effects of mastoparan on intracellular free Ca2+ concentrations in human astrocytoma cells.

机译：麦草胺对人星形细胞瘤细胞内游离钙离子浓度的双重影响。

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1. The effect of mastoparan, a wasp venom toxin, on intracellular free Ca2+ concentration ([Ca2+]i) was examined in human astrocytoma cells. Mastoparan inhibited [Ca2+]i induced by carbachol (100 microM) in a concentration-dependent manner in the absence of extracellular Ca2+, consistent with our previous results showing that mastoparan inhibits phosphoinositide hydrolysis in human astrocytoma cells. 2. In contrast, mastoparan itself increased [Ca2+]i and augmented carbachol-induced increase in the [Ca2+]i in the presence of extracellular Ca2+, suggesting that mastoparan elicited Ca2+ influx from the extracellular medium. The increase appeared to be maximum at extracellular Ca2+ concentrations of 0.1-0.2 mM. The higher concentrations of extracellular Ca2+ depressed the influx. 3. Pertussis toxin did not affect mastoparan-induced inhibition of [Ca2+]i in the absence of extracellular Ca2+, consistent with the previous results that pertussis toxin did not affect mastoparan-induced inhibition of phosphoinositide hydrolysis. 4. Pertussis toxin augmented mastoparan-induced increase in [Ca2+]i in the presence of extracellular Ca2+, suggesting that pertussis toxin substrate(s) seems to be inhibitory for Ca2+ influx induced by mastoparan. 5. Verapamil, nifedipine and diltiazem (each 10 microM), L-type Ca2+ antagonists, did not affect mastoparan-induced Ca2+ influx. However, verapamil (10 microM) slightly inhibited the increase in [Ca2+]i induced by carbachol in the presence of mastoparan. 6. The results obtained in the present study indicate that mastoparan has two opposite effects on [Ca2+]i in human astrocytoma cells and possibly has at least two sites of action.
机译：1.在人类星形细胞瘤细胞中检查了马索帕兰（一种黄蜂毒毒素）对细胞内游离Ca2 +浓度（[Ca2 +] i）的影响。在没有细胞外Ca2 +的情况下，Mastoparan以浓度依赖的方式抑制了卡巴胆碱（100 microM）诱导的[Ca2 +] i，这与我们先前的结果表明，马索帕兰抑制人星形细胞瘤细胞中的磷酸肌醇水解有关。 2.相反，在存在细胞外Ca2 +的情况下，mastopranan本身会增加[Ca2 +] i，并增加卡巴胆碱诱导的[Ca2 +] i的增加，这表明mastopranan会引起来自细胞外介质的Ca2 +流入。在细胞外Ca2 +浓度为0.1-0.2 mM时，这种增加似乎最大。较高浓度的细胞外Ca2 +抑制了涌入。 3.在不存在细胞外Ca2 +的情况下，百日咳毒素不影响帕帕斯菌对[Ca2 +] i的抑制，这与以前的结果一致，即百日咳毒素不影响帕帕斯菌对磷酸肌醇水解的抑制作用。 4.在存在细胞外Ca2 +的情况下，百日咳毒素增强了马索帕兰诱导的[Ca2 +] i的增加，这表明百日咳毒素底物似乎抑制了由马索帕兰诱导的Ca2 +内流。 5.维拉帕米，硝苯地平和地尔硫卓（各10 microM），L型Ca2 +拮抗剂，不影响马索帕兰诱导的Ca2 +流入。但是，维拉帕米（10 microM）略微抑制在存在马多巴兰的情况下卡巴胆碱诱导的[Ca2 +] i的增加。 6.在本研究中获得的结果表明，马索帕兰对人星形细胞瘤细胞中的[Ca2 +] i具有两个相反的作用，并且可能至少具有两个作用位点。

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