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An Experimental Study on Citric Acid Production by Aspergillus niger Using Gelidiella acerosa as a Substrate

机译:黑头曲霉作为底物黑曲霉生产柠檬酸的实验研究

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摘要

Citric acid (CA) is the most important commercial product which is produced by using various sugar substrates in the terrestrial environment. The present study made an attempt to produce citric acid by the fungal strain Aspergillus niger from red seaweed Gelidiella acerosa is the best alternative to sugar substrate in the marine environment. In this study three types of production media were prepared including control (sucrose) by following standard fermentation conditions. The acid production was indicated by the reduction of pH levels. The control medium gave the highest yield of 80 g/l at pH 1.5 and the medium containing crude seaweed powder and other compositions gave the yield of 30 g/l at pH 3.5 whereas the medium containing crude seaweed and 10% sucrose gave the yield of 50 g/l at pH 3.0. When calculating the benefit cost ratio, crude seaweed powder and 10% sucrose yielded 50 g of citric acid at the lower cost of Rs. 35, whereas the other two media gave the yield of 80 and 30 g respectively with the cost of Rs. 77 and 28. In economic point of view, the medium containing seaweed and 10% sucrose showed more benefit with lower cost.
机译:柠檬酸(CA)是最重要的商业产品,通过在陆地环境中使用各种糖基质生产。本研究尝试了由红海藻Geridiella acerosa的黑曲霉菌株生产柠檬酸,是海洋环境中糖底物的最佳替代品。在这项研究中,通过遵循标准的发酵条件,制备了三种类型的生产培养基,包括对照(蔗糖)。 pH值的降低表明了酸的产生。对照培养基在pH 1.5下的最高产量为80 g / l,含有粗海藻粉和其他成分的培养基在pH 3.5的情况下产量为30 g / l,而含有粗海藻和10%蔗糖的培养基的产量为50 g / l。 pH 3.0时50 g / l。在计算收益成本比率时,粗制海藻粉和10%蔗糖以较低的卢比成本产生了50克柠檬酸。 35,而其他两种媒体分别以80卢比和30卢比的成本给出了产量。 77和28.从经济角度来看,含有海藻和10%蔗糖的培养基显示出更多的好处,同时成本更低。

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