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Protective effects of acerola juice on genotoxicity induced by iron in vivo

机译:金银花汁对铁体内遗传毒性的保护作用。

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摘要

Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola juices were mutagenic to animals treated with juices, in micronucleus test. However, when compared to iron group, the pre-treatment with acerola juices exerted antimutagenic activity, decreasing significantly micronucleus mean values in bone marrow. Stage of ripeness did not influence the interaction of acerola compounds with DNA, and both ripe and unripe acerola juices exerted protective effect over DNA damage generated by iron.
机译:铁等金属离子可通过诱导活性氧(ROS)和氧化应激来诱导DNA损伤。维生素C是世界上使用最广泛的抗氧化剂之一,存在于许多水果和蔬菜中,尤其是在巴西产的被称为金虎尾的小圆叶锦葵中。金针菇由于其高抗氧化性能和酚含量而被认为是功能性水果,因此可以食用以预防疾病或在治疗策略中用作佐剂。在这里,使用彗星测定法和微核试验,在体内分析了成熟和未成熟的西印度樱桃汁对铁遗传毒性的影响。彗星试验结果表明,金虎尾汁没有任何遗传毒性或抗原毒性。在微核试验中,成熟或未成熟的西印度樱桃汁都不会对用汁液处理过的动物致突变。但是,与铁组相比,金银花汁的预处理具有抗诱变活性,可显着降低骨髓中的微核平均值。成熟阶段不影响针叶化合物与DNA的相互作用,并且成熟和未成熟的针叶汁均对铁产生的DNA损伤具有保护作用。

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