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首页> 美国卫生研究院文献>other >Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia Morganella morganii Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria
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Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia Morganella morganii Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria

机译：从尼日利亚西南部禽类分离出的肺炎克雷伯菌摩根氏菌摩登莱克氏菌和弗氏柠檬酸杆菌菌株中广谱β-内酰胺酶和质粒介导的喹诺酮抗性基因的流行

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A serious concern is arising on the coexistence of extended-spectrum beta-lactamase (ESBL) and plasmid mediated quinolone resistance (PMQR) producing bacteria in animal husbandry, which could be transferred to humans, especially in strains that may not be routinely screened for resistance. This study therefore tested the prevalence of ESBL and PMQR genes in selected bacteria isolated from poultry faeces. Faecal droppings of birds were collected from 11 farms in five states in South Western Nigeria. Bacteria were isolated from the samples on cefotaxime supplemented plates and identified with MALDI-TOF. The MIC was determined using VITEK system and resistance genes were detected with PCR. A total of 350 strains were isolated from different samples and selected strains were identified as 23 Klebsiella pneumonia, 12 Morganella morganii, seven Leclercia adecarboxylata and one Citrobacter freundii. All the species were resistant to gentamycin, trimethoprim/sulphamethaxole, tobramycin, piperacillin, cefotaxime and aztreonam (except Morganella morganii strains which were mostly susceptible to aztreonam). All the tested strains were susceptible to imipenem, meropenem and amikacin. All Leclercia adecarboxylata strains were resistant to ceftazidime, cefepime and fosfomycin while all Morganella morganii strains were resistant to fosfomycin, moxifloxacin and ciprofloxacin. All tested species were generally sensitive to ciprofloxacin except Morganella morganii strains which were resistant to ciprofloxacin. The resistance to ciprofloxacin, ceftazidime, cefepime, tigercylin, colistin and fosfomycin were 65%, 40%, 23%,, 7%, 33%, 48% respectively while the prevalence of SHV, TEM and CTX genes were 42%, 63%, 35% respectively. 9.3% of the isolates had the three ESBL genes, 2.33% had qnrA gene, 4.65% had qnr B gene while none had qnrS gene. The most prevalent PMQR gene is Oqxb (25.58%) while 6.98% had the qep gene. Klebsiella pneumoniae generally had both ESBL and PMQR genes. The high prevalence of extended spectrum beta-lactamase genes in the studied strains calls for caution in the use of beta lactam antibiotics in poultry feeds. This is the first report of the occurrence of extended spectrum beta-lactamase and plasmid mediated quinolone resistance genes in Morganella morganii and Leclercia adecarboxylata strains isolated from poultry faeces.
机译：在畜牧业中，广谱β-内酰胺酶（ESBL）和质粒介导的喹诺酮抗药性（PMQR）产生细菌的共存引起了人们的严重关注，这些细菌可能会转移给人类，尤其是在可能无法常规筛查抗药性的菌株中。因此，这项研究测试了从家禽粪便中分离出的选定细菌中ESBL和PMQR基因的普遍性。从尼日利亚西南部五个州的11个农场收集粪便粪便。在补充头孢噻肟的板上从样品中分离细菌，并用MALDI-TOF鉴定。使用VITEK系统确定MIC，并通过PCR检测抗性基因。从不同样品中共分离出350株，选择的菌株被鉴定为23株肺炎克雷伯菌，12株摩根氏摩根氏菌，7株嗜酸乳杆菌和1株弗氏柠檬酸杆菌。所有物种均对庆大霉素，甲氧苄氨嘧啶/磺胺甲氧嘧啶，妥布霉素，哌拉西林，头孢噻肟和氨曲南具有抗药性（摩根氏菌（Morganella morganii）菌株最容易受到氨曲南的影响）。所有测试的菌株均对亚胺培南，美罗培南和丁胺卡那霉素敏感。所有莱德氏菌嗜酸乳杆菌菌株均对头孢他啶，头孢吡肟和磷霉素具有抗性，而所有摩根摩根氏菌菌株均对磷霉素，莫西沙星和环丙沙星具有抗性。除了对环丙沙星具有抗性的摩根氏菌之外，所有测试物种通常对环丙沙星敏感。对环丙沙星，头孢他啶，头孢吡肟，虎胆碱，粘菌素和磷霉素的耐药率分别为65％，40％，23％，7％，33％，48％，而SHV，TEM和CTX基因的患病率分别为42％，63％ ，分别为35％。分离株中有9.3％具有三个ESBL基因，2.33％具有qnrA基因，4.65％具有qnr B基因，而没有qnrS基因。最普遍的PMQR基因是Oqxb（25.58％），而6.98％具有qep基因。肺炎克雷伯菌一般都具有ESBL和PMQR基因。在所研究的菌株中，广谱β-内酰胺酶基因的高流行率要求在禽饲料中使用β-内酰胺抗生素时要谨慎。这是首次报道从家禽粪便中分离出的摩根氏摩根氏菌和德莱克氏菌菌株中存在广谱β-内酰胺酶和质粒介导的喹诺酮抗性基因。

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